Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Standardization of Alternaria alternata: Extraction and quantification of Alt a 1 by using an mAb-based 2-site binding assay
Autore:
Aden, E; Weber, B; Bossert, J; Teppke, M; Frank, E; Wahl, R; Fiebig, H; Cromwell, O;
Indirizzi:
Allergopharma Joachim Ganzer KG, D-21465 Reinbek, Germany Allergopharma Joachim Ganzer KG Reinbek Germany D-21465 Reinbek, Germany Univ Hamburg, Dept Biochem & Mol Biol, Hamburg, Germany Univ Hamburg Hamburg Germany Dept Biochem & Mol Biol, Hamburg, Germany
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 1, volume: 104, anno: 1999,
pagine: 128 - 135
SICI:
0091-6749(199907)104:1<128:SOAAEA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; MOLECULAR-CLONING; MAJOR ALLERGENS; IGE ANTIBODIES; DERMATOPHAGOIDES; PROTEINS; ELISA;
Keywords:
Alternaria alternata; monoclonal antibody; Alt a 1; ELISA; skin prick test; allergens; fungi;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Weber, B Allergopharma Joachim Ganzer KG, Hermann Korner Str 52, D-21465 Reinbek, Germany Allergopharma Joachim Ganzer KG Hermann Korner Str 52 Reinbek Germany D-21465
Citazione:
E. Aden et al., "Standardization of Alternaria alternata: Extraction and quantification of Alt a 1 by using an mAb-based 2-site binding assay", J ALLERG CL, 104(1), 1999, pp. 128-135

Abstract

Background: Alternaria alternata is recognized as an important cause of allergic disease. As with other molds, the extracts of A alternata used for diagnosis and therapy are highly heterogeneous, and there is a need for improved standardization. The major allergen Alt a 1 is well characterized and has been produced as a recombinant protein, but very few data are availableon the Alt a 1 content in extracts. Objective: An assay for the quantification of Alt a 1 was developed and used for monitoring batch-to-batch consistency of A alternata extracts, and the correlation between skin prick test responses and Alt a 1 concentrationswas studied. Methods: A 2-site binding assay based on an Alt a 1-specific mAb was developed and used for the quantification of Alt a 1 in allergen extracts. Quantitative skin prick tests were performed on 16 A alternata-sensitive patients and correlated with the Alt a 1 concentration. Results: The Alt a 1-specific mAb was found to be suitable for affinity purification, as well as for a 2-site binding quantification assay. In allergen extracts the Alt a 1 content was estimated as 2% to 4.7% of total protein. Quantitative skin prick tests showed an Alt a 1 concentration-dependent response. Conclusion: Quantification of Alt a 1 in A alternata extracts reflects their batch-to-batch consistency Skin prick test responses to a standardized Aalternata extract correlate with the Alt a 1 contents. An extract containing 3.7 mu g/mL Alt a 1 caused a response equal to that of a 1% histamine dihydrochloride solution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:45:22