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Titolo:
Oxidative stress leads to cholesterol accumulation in vascular smooth muscle cells
Autore:
Gesquiere, L; Loreau, N; Minnich, A; Davignon, J; Blache, D;
Indirizzi:
Univ Bourgogne, Fac Med, INSERM, U498, F-21033 Dijon, France Univ Bourgogne Dijon France F-21033 INSERM, U498, F-21033 Dijon, France Rhone Poulenc Rorer, Res & Dev, Collegeville, PA USA Rhone Poulenc Rorer Collegeville PA USA Res & Dev, Collegeville, PA USA Clin Res Inst Montreal, Hyperlipidemia & Atherosclerosis Res Grp, Montreal, PQ H2W 1R7, Canada Clin Res Inst Montreal Montreal PQ Canada H2W 1R7 eal, PQ H2W 1R7, Canada
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 1-2, volume: 27, anno: 1999,
pagine: 134 - 145
SICI:
0891-5849(199907)27:1-2<134:OSLTCA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; HERITABLE HYPERLIPIDEMIC RABBIT; MACROPHAGE FOAM CELLS; ACYL-COENZYME-A; FAMILIAL HYPERCHOLESTEROLEMIA; LIPID-PEROXIDATION; ESTER CYCLE; FATTY-ACIDS; VITAMIN-E; ATHEROSCLEROSIS;
Keywords:
free radicals; vascular smooth muscle cells; cholesterol biosynthesis; cholesterol efflux; antioxidants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Blache, D Univ Bourgogne, Fac Med, INSERM, U498, 7 Bd Jeanne dArc, F-21033Dijon, France Univ Bourgogne 7 Bd Jeanne dArc Dijon France F-21033 on, France
Citazione:
L. Gesquiere et al., "Oxidative stress leads to cholesterol accumulation in vascular smooth muscle cells", FREE RAD B, 27(1-2), 1999, pp. 134-145

Abstract

The transformation of macrophages and smooth muscle cells into foam cells by modified low-density lipoproteins (LDL) is one of the key events of atherogenesis. Effects of free radicals have mainly been studied in LDL, and other than toxicity, data dealing with direct action of free radicals on cells are scarce. This study focused on the direct effects of free radicals on cholesterol metabolism of smooth muscle cells. A free radical generator, azobisamidinopropane dihydrochloride, was used, and conditions for a standardized oxidative stress were set up in vascular smooth muscle cells. After free radical action, the cells presented an accumulation of cholesterol that appeared to be the result of: (i) an increase in cholesterol biosynthesis and esterification; (ii) a decrease in cell cholesteryl ester hydrolysis; and (iii) a reduced cholesterol efflux. All these parameters were opposed by antioxidants. In addition, oxidant stress induced an increased degradation of acetyl-LDL, whereas no change was noted for native LDL. From this data, it was concluded that cholesterol metabolism of vascular smooth muscle cells was markedly altered by in vitro treatment with free radicals, although cell viability was unaffected. The resulting disturbance in cholesterol metabolism favors accumulation of cholesterol and cholesteryl esters in vascular cells, and thus may contribute to the formation of smooth muscle foam cells. (C) 1999 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:09:29