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Titolo:
Influence of extracellular matrix overlay and medium formulation on the induction of cytochrome P-4502B enzymes in primary cultures of rat hepatocytes
Autore:
Lecluyse, E; Bullock, P; Madan, A; Carroll, K; Parkinson, A;
Indirizzi:
XenoTech LLC, Kansas City, KS 66103 USA XenoTech LLC Kansas City KS USA 66103 Tech LLC, Kansas City, KS 66103 USA Univ Kansas, Med Ctr, Ctr Environm & Occupat Hlth, Dept Pharmacol Toxicol & Therapeut, Kansas City, KS 66103 USA Univ Kansas Kansas City KS USA 66103 Therapeut, Kansas City, KS 66103 USA
Titolo Testata:
DRUG METABOLISM AND DISPOSITION
fascicolo: 8, volume: 27, anno: 1999,
pagine: 909 - 915
SICI:
0090-9556(199908)27:8<909:IOEMOA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLLAGEN-SANDWICH CONFIGURATION; MICROSOMAL CYTOCHROME-P-450; LIVER; PURIFICATION; EXPRESSION; ANTIBODIES; DEPENDENCE; INVITRO; CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Madan, A XenoTech LLC, 3800 Cambridge, Kansas City, KS 66103 USA XenoTech LLC 3800 Cambridge Kansas City KS USA 66103 KS 66103 USA
Citazione:
E. Lecluyse et al., "Influence of extracellular matrix overlay and medium formulation on the induction of cytochrome P-4502B enzymes in primary cultures of rat hepatocytes", DRUG META D, 27(8), 1999, pp. 909-915

Abstract

The effect of medium formulation, composition of extracellular matrix overlay, and culture dish material on liver microsomal cytochrome P-450 (CYP) 2B induction by phenobarbital (PB) was investigated in primary cultures of rat hepatocytes. When hepatocytes were maintained on Permanox dishes with anoverlay of either collagen (type I) or Matrigel, Williams' E medium was superior to other medium formulations in terms of the magnitude of induction of CYP2B on a per milligram microsomal protein basis. Modified Chee's medium (MCM) and hepatocyte culture medium were intermediate in their capacity to sustain induction of CYP2B by PB, and Dulbecco's modified Eagle's medium was slightly less effective. The overall induction of CYP2B activity by PB was, on average, 50% lower in hepatocytes cultured on polystyrene dishes (LUX), Little or no difference was observed between hepatocytes overlaid withcollagen and those overlaid with Matrigel, MCM was superior to Williams' Emedium in terms of the yield of microsomal protein and the ultrastructuralfeatures of the hepatocyte monolayers. CYP2B induction by PB was optimal after 3 days of treatment in either medium. CYP1A, CYP3A, and CYP4A activities could be induced in vitro by prototypical inducing agents in hepatocytescultured on Permanox dishes with MCM and a Matrigel overlay to comparable levels observed in vivo. The results of these studies show that medium formulation and culture vessel material, but not the type of extracellular matrix overlay, have significant effects on the induction of CYP enzymes in cultured rat hepatocytes maintained in a sandwich configuration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 21:59:22