Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Embryonic mesenchymal cells share the potential for smooth muscle differentiation: myogenesis is controlled by the cell's shape
Autore:
Yang, Y; Relan, NK; Przywara, DA; Schuger, L;
Indirizzi:
Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 Dept Pathol, Detroit, MI 48201 USA Wayne State Univ, Sch Med, Dept Pharmacol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 pt Pharmacol, Detroit, MI 48201 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 13, volume: 126, anno: 1999,
pagine: 3027 - 3033
SICI:
0950-1991(199907)126:13<3027:EMCSTP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR-BETA-1; ACTIN EXPRESSION; MOUSE EMBRYOGENESIS; LUNG DEVELOPMENT; RAT LUNG; ACTIVATION; FIBRONECTIN; INDUCTION; LINEAGES; ISOFORM;
Keywords:
smooth muscle; differentiation; myogenesis; cell shape; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Schuger, L Wayne State Univ, Sch Med, Dept Pathol, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 l, Detroit, MI 48201 USA
Citazione:
Y. Yang et al., "Embryonic mesenchymal cells share the potential for smooth muscle differentiation: myogenesis is controlled by the cell's shape", DEVELOPMENT, 126(13), 1999, pp. 3027-3033

Abstract

Undifferentiated embryonic mesenchymal cells are round/cuboidal in shape. During development, visceral myogenesis is shortly preceded by mesenchymal cell elongation. To determine the role of the cell's shape on smooth muscledevelopment, undifferentiated embryonic mesenchymal cells from intestine (abundant visceral muscle), lung (some visceral muscle) or kidney (no visceral muscle) were plated under conditions that maintained cell rounding or promoted elongation. Regardless of their fate in vivo, all the cells differentiated into smooth muscle upon elongation as indicated by the expression ofsmooth muscle-specific proteins and the development of membrane potentialsof -60 mV and voltage-dependent Ca2+ currents, characteristic of excitablecells. Smooth muscle differentiation occurred within 24 hours and was independent of cell proliferation. Regardless of their fate in vivo, all the round cells remained negative for smooth muscle markers, had membrane potentials of -30 mV and showed no voltage-activated current, These cells, however, differentiated into smooth muscle upon elongation. The role of the cell'sshape in controlling smooth muscle differentiation was not overcome by treatment with retinoic acid, TGF-beta 1, PDGF BE or epithelial-conditioned medium (all modulators of smooth muscle differentiation). These studies suggest that the mesenchymal cell shape plays a main role in visceral myogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:56:17