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Titolo:
READING-FRAME RESTORATION BY TRANSCRIPTIONAL SLIPPAGE AT LONG STRETCHES OF ADENINE RESIDUES IN MAMMALIAN-CELLS
Autore:
LINTON MF; RAABE M; PIEROTTI V; YOUNG SG;
Indirizzi:
VANDERBILT UNIV,MED CTR,DIV ENDOCRINOL NASHVILLE TN 37232 VANDERBILT UNIV,SCH MED,DEPT MED NASHVILLE TN 37232 VANDERBILT UNIV,SCH MED,DEPT PHARMACOL NASHVILLE TN 37232 UNIV CALIF SAN FRANCISCO,GLADSTONE INST CARDIOVASC DIS SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST SAN FRANCISCO CA 94141 UNIV CALIF SAN FRANCISCO,DEPT MED SAN FRANCISCO CA 94141
Titolo Testata:
The Journal of biological chemistry
fascicolo: 22, volume: 272, anno: 1997,
pagine: 14127 - 14132
SICI:
0021-9258(1997)272:22<14127:RRBTSA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-III HOLOENZYME; APOLIPOPROTEIN-B GENE; FAMILIAL HYPOBETALIPOPROTEINEMIA; PLASMA-CONCENTRATIONS; ESCHERICHIA-COLI; GAMMA-SUBUNIT; DNA; EXPRESSION; DENSITY; B100;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
M.F. Linton et al., "READING-FRAME RESTORATION BY TRANSCRIPTIONAL SLIPPAGE AT LONG STRETCHES OF ADENINE RESIDUES IN MAMMALIAN-CELLS", The Journal of biological chemistry, 272(22), 1997, pp. 14127-14132

Abstract

We previously characterized a mutant apoB allele (the apoB86 allele) that produces both a truncated apoB (apoB86) and a full length apoB100, The mutant allele contained a deletion of a single cytosine in exon 26, creating a stretch of eight consecutive adenines in the -1 readingframe. The altered reading-frame allele was restored, with similar to10% efficiency, by the transcriptional insertion of an extra adenine into the stretch of eight consecutive adenines, thereby accounting forthe synthesis of the full-length apoB100, Here, we demonstrate that this reading-frame restoration does not occur when the long stretch of adenines is interrupted by a cytosine, To assess whether reading-framerestoration is unique to a single site in the apoB gene, the same mutation (eight consecutive adenines in the -1 reading frame) was inserted into another site within the apoB gene, Reading-frame restoration occurred at the second site and was abrogated when the stretch of adenines was interrupted by another base, Of note, a computerized analysis of human cDNA sequences revealed that long stretches of adenines in protein-coding sequences occur at a lower than predicted frequency, suggesting that evolution has selected against these sequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:53:07