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Titolo:
Regulation of osteogenic differentiation of human bone marrow stromal cells: Interaction between transforming growth factor-beta and 1,25(OH)(2) vitamin D-3 in vitro
Autore:
Liu, P; Oyajobi, BO; Russell, RGG; Scutt, A;
Indirizzi:
Univ Sheffield, Sch Med, Div Biochem & Musculoskeletal Med, Sheffield S10 2RX, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S102RX S Yorkshire, England
Titolo Testata:
CALCIFIED TISSUE INTERNATIONAL
fascicolo: 2, volume: 65, anno: 1999,
pagine: 173 - 180
SICI:
0171-967X(199908)65:2<173:ROODOH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEOBLAST-LIKE CELLS; 1,25-DIHYDROXYVITAMIN D-3; ALKALINE-PHOSPHATASE; ANABOLIC ACTIONS; PRECURSOR CELLS; TGF-BETA; CFU-F; RATS; INCREASES; INVITRO;
Keywords:
human bone marrow stromal cells; TGF-beta; 1,25(OH)(2)D-3; osteogenic differentiation; osteogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Scutt, A Univ Connecticut, Ctr Hlth, Dept Pediat, Farmington, CT 06032 USAUniv Connecticut Farmington CT USA 06032 armington, CT 06032 USA
Citazione:
P. Liu et al., "Regulation of osteogenic differentiation of human bone marrow stromal cells: Interaction between transforming growth factor-beta and 1,25(OH)(2) vitamin D-3 in vitro", CALCIF TIS, 65(2), 1999, pp. 173-180

Abstract

Bone marrow stromal cells are believed to play a major role in bone formation as a major source of osteoprogenitor cells, however, very little is known about how the osteogenic differentiation of these cells is regulated by systemic hormones and local growth factors. We examined the effects of TGF-beta and its interaction with 1,25(OH)(2) Vitamin D-3 [1,25(OH)(2)D-3] on the differentiation and proliferation of human bone marrow stromal cells (hBMSC) in secondary cultures. Alkaline phosphatase (ALP) activity was inhibited by TGF-beta (0.1-10 ng/ml) and increased by 1,25(OH)(2)D-3 (50 nM), however, co-treatment of TGF-beta and 1,25(OH)(2)D-3 synergistically enhanced ALP activity with maximal stimulation occurring at about 8 days after treatment. This synergistic effect was independent of proliferation because, in contrast to TGF-beta alone, combined treatment with TGF-beta and 1,25(OH)(2)D-3 had no effect on hBMSC proliferation. As no synergistic effect was seenwith combinations of 1,25(OH)(2)D-3 and other osteotrophic growth factors,including BMP-2, IGF-I, and basic fibroblast growth factor (bFGF), it would seem likely that the synergistic interaction is specific for TGF-beta. The increased ALP activity was due to an enhancement of 1,25(OH)(2)D-3-induced ALP activity by TGF-beta, rather than vice versa. In contrast, TGF-beta inhibited 1,25(OH)(2)D-3-induced osteocalcin production. Taken together, these results indicate that TGF-beta and 1,25(OH)(2)D-3 act synergistically tostimulate the recruitment of BMSC to the osteoblast lineage. This interaction may play an important role in bone remodeling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:14:48