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Titolo:
The soluble interleukin-6 (IL-6) receptor/IL-6 fusion protein enhances in vitro maintenance and proliferation of human CD34(+)CD38(-/low) cells capable of repopulating severe combined immunodeficiency mice
Autore:
Kollet, O; Aviram, R; Chebath, J; ben-Hur, H; Nagler, A; Shultz, L; Revel, M; Lapidot, T;
Indirizzi:
Weizmann Inst Sci, Pauline Recanati Career Dev Chair Immunol, Dept Immunol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 unol, IL-76100 Rehovot, Israel Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 enet, IL-76100 Rehovot, Israel Kaplan Hosp, Dept Obstet & Gynecol, IL-76100 Rehovot, Israel Kaplan Hosp Rehovot Israel IL-76100 & Gynecol, IL-76100 Rehovot, Israel Hadassah Univ Hosp, Dept Bone Marrow Transplant, IL-91120 Jerusalem, Israel Hadassah Univ Hosp Jerusalem Israel IL-91120 IL-91120 Jerusalem, Israel Jackson Lab, Bar Harbor, ME 04609 USA Jackson Lab Bar Harbor ME USA 04609Jackson Lab, Bar Harbor, ME 04609 USA
Titolo Testata:
BLOOD
fascicolo: 3, volume: 94, anno: 1999,
pagine: 923 - 931
SICI:
0006-4971(19990801)94:3<923:TSI(RF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; BONE-MARROW STROMA; HUMAN CORD-BLOOD; C-KIT LIGAND; IN-VITRO; PROGENITOR CELLS; FLT3 LIGAND; STEM-CELLS; CYTOKINE; GP130;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Lapidot, T Weizmann Inst Sci, Pauline Recanati Career Dev Chair Immunol, Dept Immunol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 100 Rehovot, Israel
Citazione:
O. Kollet et al., "The soluble interleukin-6 (IL-6) receptor/IL-6 fusion protein enhances in vitro maintenance and proliferation of human CD34(+)CD38(-/low) cells capable of repopulating severe combined immunodeficiency mice", BLOOD, 94(3), 1999, pp. 923-931

Abstract

In vitro maintenance and proliferation of human hematopoietic stem cells is crucial for many clinical applications. Early hematopoietic cells expresslow levels of FLT-3 and c-kit receptors, as well as the interleukin-6 (IL-6) receptor signal transducing element, gp130, but do not express IL-6 receptor itself. Therefore, we have attempted to maintain human cord blood or bone marrow CD34(+) cells ex vivo in serum-free cultures containing stem cell factor (SCF) and FLT-3 ligand (FL) alone or together with a new recombinant molecule of soluble IL-6 receptor fused to IL-6 (IL6RIL6 chimera). The effect of IL6RIL6 chimera on the proliferation and differentiation of CD34(+) cells was compared with that of each chimera component added separately, The engraftment potential of in vitro-cultured cells was determined using our recently established functional in vivo assay for primitive human severecombined immunodeficiency (SCID)-repopulating cells (SRC), We report here that IL6RIL6 chimera induced significantly higher levels of progenitors andSRC compared with SCF + FL alone or together with IL-6 and soluble IL-6 receptor. IL6RIL6 chimera prolonged in vitro maintenance of SRC for up to 14 days. Stimulation of CD34(+)CD38(-/low) enriched cells with IL6RIL6 chimeramaintained the early CD34(+)CD38(-/low) cell subpopulation, which could bedetected in vitro for up to 14 days. Moreover, IL6RIL6 chimera preferentially stimulated the growth of early CD34(+)38(-/low) cells, resulting in significantly higher levels of progenitors compared with more mature CD34(+)38(+) cells. Taken together, these findings demonstrate the importance of IL6RIL6 chimera in stimulating the proliferation of early CD34(+) CD38(-)gp130(+)IL-6R(-) cells in vitro and extended maintenance of progenitors and SRC,(C) 1999 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:35:49