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Titolo:
Lymphocyte populations in tuberculous pleural effusions
Autore:
San Jose, ME; Valdes, L; Saavedra, MJ; De Vega, JM; Alvarez, D; Vinuela, J; Penela, P; Valle, JM; Seoane, R;
Indirizzi:
Complejo Hosp Univ Santiago, Hosp Conxo, Serv Anal Clin, Santiago 15706, La Coruna, Spain Complejo Hosp Univ Santiago Santiago La Coruna Spain 15706 Coruna, Spain Complejo Hosp Univ Santiago, Hosp Conxo, Serv Neumol, Santiago 15706, La Coruna, Spain Complejo Hosp Univ Santiago Santiago La Coruna Spain 15706 Coruna, Spain Complejo Hosp Univ Santiago, Hosp Conxo, Serv Inmunol, Santiago 15706, La Coruna, Spain Complejo Hosp Univ Santiago Santiago La Coruna Spain 15706 Coruna, Spain
Titolo Testata:
ANNALS OF CLINICAL BIOCHEMISTRY
, volume: 36, anno: 1999,
parte:, 4
pagine: 492 - 500
SICI:
0004-5632(199907)36:<492:LPITPE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINE-DEAMINASE ACTIVITY; MYCOBACTERIUM-TUBERCULOSIS; ALVEOLAR MACROPHAGES; SERUM; CELLS; GAMMA;
Keywords:
pleuritis; tuberculosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: San Jose, ME Complejo Hosp Univ Santiago, Hosp Conxo, Serv Anal Clin, Rua Ramon Baltar S-N, Santiago 15706, La Coruna, Spain Complejo Hosp Univ Santiago Rua Ramon Baltar S-N Santiago La Coruna Spain 15706
Citazione:
M.E. San Jose et al., "Lymphocyte populations in tuberculous pleural effusions", ANN CLIN BI, 36, 1999, pp. 492-500

Abstract

Different systemic and local responses to mycobacterial antigens suggest an active compartmentalization of responsive lymphocytes to tubercular antigens. This fact, observed in pleuritic processes, raises doubts about the accuracy of information obtained in the study of cells taken solely from peripheral blood. For this reason we decided to study the concept of compartmentalization in 140 patients suffering from pleural effusions. Patients were classified into six groups according to the aetiology of the effusion: group I, tuberculous, n = 23; group II, paraneoplastic, n = 41; group III, metapneumonic empyematous, n = 5; group IV, transudate, n = 38; group V, miscellaneous exudate, n = 19; group VI, unknown aetiology, n = 14. In each groupwe studied the lymphocyte population by using flow cytometry with doubly fluorescent monoclonal antibodies: B [expressing human lymphocyte antigen (HLA)-DR on the surface], T (CD3 +), CD4 + and CD8 +, and the subpopulation of activated T lymphocytes (together expressing CD3 and HLA-DR on the surface) (CD3 + DR +). The study of these subpopulations in peripheral blood did not yield valuable results, but the CD3 + DR + population in pleural fluid demonstrated a diagnostic efficiency of 84% [positive predictive value (PPV) 51%, negative predictive value (NPV) 96%] at a cut-off value of 80.4 cells/mm(3). The CD3 + DR + pleural fluid/peripheral blood ratio demonstrated an efficiency of 83% (PPV 50%, NPV 96%), and showed a statistically significant difference (P < 0.02) with regard to all the diagnostic groups, with the exception of the paraneoplastic effusions. The lymphocytic subpopulationsstudy confirms the concept of compartmentalization in tuberculous pleuritis, as shown by the greater number of activated T lymphocytes present in pleural fluid in comparison with peripheral blood in tuberculous pleuritis, a 98% efficiency of adenosine deaminase (ADA) determination in pleural fluid versus a 50% value in peripheral blood, predominance of helper cells (CD4 +) in pleural fluid and suppressor cells (CD8 +) in peripheral blood, a greater CD4 +/CD8 + ratio in pleural fluid than in peripheral blood, and a significant correlation of ADA-CD3 + DR + in pleural fluid, which does not occur in peripheral blood.

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Documento generato il 23/11/20 alle ore 21:36:21