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Titolo:
Determination of sparfloxacin in plasma and urine by a simple and rapid liquid chromatographic method
Autore:
Kamberi, M; Kamberi, P; Hajime, N; Uemura, N; Nakamura, K; Nakano, S;
Indirizzi:
Oita Med Univ, Dept Clin Pharmacol & Therapeut, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 rmacol & Therapeut, Oita 8795593, Japan Oita Med Univ, Dept Internal Med 2, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 ept Internal Med 2, Oita 8795593, Japan
Titolo Testata:
THERAPEUTIC DRUG MONITORING
fascicolo: 4, volume: 21, anno: 1999,
pagine: 411 - 415
SICI:
0163-4356(199908)21:4<411:DOSIPA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Keywords:
sparfloxacin; plasma; urine; high-performance liquid chromatography; ultraviolet detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Kamberi, M Oita Med Univ, Dept Clin Pharmacol & Therapeut, 1-1 Hasama Machi, Oita 8795593, Japan Oita Med Univ 1-1 Hasama Machi Oita Japan 8795593 95593, Japan
Citazione:
M. Kamberi et al., "Determination of sparfloxacin in plasma and urine by a simple and rapid liquid chromatographic method", THER DRUG M, 21(4), 1999, pp. 411-415

Abstract

A simple, specific, sensitive, and rapid method has been developed and validated for the determination of sparfloxacin in human plasma and urine. Theassay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were efficiently removed by precipitation with perchloric acid after the addition of grepafloxacin as an internal standard, For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C-18 reversed-phase column. The quantification limit was 0.025 mg/L in plasma and 0.5 mg/L in urine. The coefficients of variation (CV) were less than 10% for intra-day and inter-day analyses. The recovery of sparfloxacin added to plasma and urine ranged from 96.7% to 97.9%. The method has been successfully applied to pharmacokinetic studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:21:02