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Titolo:
The effects of tolaasin, the toxin produced by Pseudomonas tolaasii on tyrosinase activities and the induction of browning in Agaricus bisporus fruiting bodies
Autore:
Soler-Rivas, C; Arpin, N; Olivier, JM; Wichers, HJ;
Indirizzi:
DLO, AT, Agrotechnol Res Inst, NL-6708 PD Wageningen, Netherlands DLO Wageningen Netherlands NL-6708 PD NL-6708 PD Wageningen, Netherlands Univ Lyon 1, Lab Mycochim, Unite Format Rech Chim & Biochim, F-69622 Villeurbanne, France Univ Lyon 1 Villeurbanne France F-69622 im, F-69622 Villeurbanne, France INRA, Stn Rech Champignons, Ctr Rech Bordeaux, F-33883 Villenave Dornon, France INRA Villenave Dornon France F-33883 x, F-33883 Villenave Dornon, France
Titolo Testata:
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
fascicolo: 1, volume: 55, anno: 1999,
pagine: 21 - 28
SICI:
0885-5765(199907)55:1<21:TEOTTT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPODEPSIPEPTIDE TOXIN; CULTIVATED MUSHROOM; BLOTCH DISEASE; IDENTIFICATION; DISCOLORATION; PROTEINS; GINGERI; SURFACE; STRAINS; OXIDASE;
Keywords:
tyrosinase; Agaricus bisporus; Pseudomonas tolaasii; tolaasin; phenols; tyrosine; GHB; GDHB; melanin; brown blotch disease; browning; discolouration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Soler-Rivas, C DLO, AT, Agrotechnol Res Inst, Bornsesteeg 59, NL-6708 PD Wageningen, Netherlands DLO Bornsesteeg 59 Wageningen Netherlands NL-6708 PD lands
Citazione:
C. Soler-Rivas et al., "The effects of tolaasin, the toxin produced by Pseudomonas tolaasii on tyrosinase activities and the induction of browning in Agaricus bisporus fruiting bodies", PHYSL MOL P, 55(1), 1999, pp. 21-28

Abstract

Infection of Agaricus bisporus with Pseudomonas tolaasii or treatment witha tolaasin containing preparation resulted in the activation of tyrosinaseand the development of a brown discolouration of the fruitbody. In order to investigate whether tolaasin is responsible for these reactions, mushrooms were treated with different concentrations of HPLC-purified tolaasin. Tolaasin was found to induce both browning and the activation of tyrosinase, with the level of activation being related to the concentration of toxin applied. In water-treated controls, active tyrosinase isoforms with pIs of 4.5and 4.4 were the main isoforms found but tolaasin treatment resulted in the activation of a tyrosinase isoform with a pi of 5.7. The different activeisoforms could be distinguished as the tolaasin activated isoforms had a higher affinity for catechol as a substrate (K-m 0.82 mM) than the active isoforms in the control (K-m 2.86 mM). In mushrooms inoculated with P. tolaasii or treated with tolaasin containing extracts no significant differences were found in the mechanism of oxidation of tyrosine, gamma-glutaminyl-4-hydroxybenzene or gamma-glutaminyl-3,4-dihydroxybenzene to form melanin. In tolaasin treated samples, a constant total amount of melanin and sum of the three phenols was found. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 16:04:31