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Titolo:
The mechanism of activation of NK-cell IFN-gamma production by ligation ofCD28
Autore:
Cheung, JC; Koh, CY; Gordon, BE; Wilder, JA; Yuan, D;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Pathol, Lab Mol Pathol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 thol, Lab Mol Pathol, Dallas, TX 75235 USA Carolina Med Ctr, Dept Comparat Med, Charlotte, NC 28232 USA Carolina Med Ctr Charlotte NC USA 28232 arat Med, Charlotte, NC 28232 USA
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 6, volume: 36, anno: 1999,
pagine: 361 - 372
SICI:
0161-5890(199904)36:6<361:TMOAON>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; MESSENGER-RNA STABILITY; MONOCLONAL-ANTIBODY; TUMOR-CELLS; CHAIN GENES; T-CELLS; RECEPTOR; COSTIMULATION; PROLIFERATION; INTERFERON;
Keywords:
NK cells; CD28; IFN-gamma; mRNA stability; Fc gamma RIII; IL-12;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Yuan, D Univ Texas, SW Med Ctr, Dept Pathol, Lab Mol Pathol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 b Mol Pathol, Dallas, TX 75235 USA
Citazione:
J.C. Cheung et al., "The mechanism of activation of NK-cell IFN-gamma production by ligation ofCD28", MOL IMMUNOL, 36(6), 1999, pp. 361-372

Abstract

We have investigated the mechanism by which anti-CD28 antibodies activatesIFN-gamma production by murine NK cells. These studies reveal that engagement of CD28 alone by this antibody is a poor activator of this cytokine response. Effective stimulation requires simultaneous ligation of the receptorfor Fc (Fc gamma RIII, CD16) which on its own is also a poor inducer of murine NK cells. The mechanism by which immobilized anti-CD28 increases IFN-gamma mRNA abundance involves both upregulation of transcription as well as induction of mRNA stabilization. However, the elevation of transcription isnot as evident as that induced by IL-12 which, in contrast, does not induce message stabilization. Thus ligation of CD28 in the presence of IL-12 results in a synergistic increase in production of the cytokine. Using this assay we have also determined that immobilized anti-CD28 cannot induce resting NK cells to produce IFN-gamma. In contrast, the same cells can be inducedby BCL1-C11 tumor cells that express high amounts of the CD28 ligand, B7-2. These studies provide important insights into the ability of cells bearing counter-receptor for CD28 to activate NK cell-cytokine production in vivo. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:51:58