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Titolo:
Oestrogen receptor (ER)-alpha and ER-beta isoforms in normal endometrial and endometriosis-derived stromal cells
Autore:
Brandenberger, AW; Lebovic, DI; Tee, MK; Ryan, IP; Tseng, JF; Jaffe, RB; Taylor, RN;
Indirizzi:
Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, Ctr Reprod Endocrinol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 7, volume: 5, anno: 1999,
pagine: 651 - 655
SICI:
1360-9947(199907)5:7<651:OR(AEI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ESTROGEN-RECEPTOR; PROGESTERONE RECEPTORS; EXPRESSION; ALPHA; TISSUE; PROLIFERATION; ACTIVATION; CYCLE; RNA; DNA;
Keywords:
cell cultures; endometriosis; oestrogen receptors alpha and beta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, RN Univ Calif San Francisco, Dept Obstet Gynecol & Reprod Sci, CtrReprod Endocrinol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 94143 USA
Citazione:
A.W. Brandenberger et al., "Oestrogen receptor (ER)-alpha and ER-beta isoforms in normal endometrial and endometriosis-derived stromal cells", MOL HUM REP, 5(7), 1999, pp. 651-655

Abstract

Several investigators have noted that hormone-dependent development of endometriosis implants lags behind that of simultaneously analysed eutopic endometrium. With the recent discovery of the oestrogen receptor-beta (ER-beta) isoform, the aim of this study was to investigate whether differences in the expression of ER-alpha and ER-beta might explain this observation. mRNAtranscripts from endometrial stromal cells isolated from normal endometrium (NE) and from endometriomas (El) were analysed using a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. RT-PCR and Southern blot analyses of the two ER isoforms indicated that NE and El stromal cells predominantly express ER-alpha mRNA, however the relative concentrations of ER isoform mRNA transcripts differed between the two cell types. Steady-state ER-alpha:ER-beta mRNA ratios were 15.5 +/- 2.8 and 5.2 +/- 0.9 respectively for NE and El cells (P = 0.02). NE and El stromal cells expressed ER proteins with similar Kd ( similar to 0.9 nM) and densities similar to 24 500 binding sites/cell respectively. Functional ER expression was indicated by an increase in progesterone receptor concentrations of similar to 60% (P = 0.03) after incubation with 10 nM oestradiol. We postulate that differential transcript processing, ligand specificity and biological actions of the ER-alpha and -beta isoforms may influence differential growth responses in normal and ectopic endometrium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:09:26