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Titolo:
Molecular cloning of the arylalkylamine-N-acetyltransferase and daily variations of its mRNA expression in the Syrian hamster pineal gland
Autore:
Gauer, F; Poirel, VJ; Garidou, ML; Simonneaux, V; Pevet, P;
Indirizzi:
Univ Strasbourg 1, CNRS, UMR 7518, F-67000 Strasbourg, France Univ Strasbourg 1 Strasbourg France F-67000 , F-67000 Strasbourg, France
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 71, anno: 1999,
pagine: 87 - 95
SICI:
0169-328X(19990723)71:1<87:MCOTAA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXYINDOLE-O-METHYLTRANSFERASE; MELATONIN SYNTHESIS; RAT PINEAL; SUPRACHIASMATIC NUCLEUS; NOCTURNAL INCREASE; CIRCADIAN-RHYTHM; GENE; RADIOIMMUNOASSAY; PHOTOPERIODS; EC-2.3.1.87;
Keywords:
pineal gland; melatonin; N-acetyltransferase; AA-NAT; in situ hybridization; Syrian hamster;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Gauer, F Univ Strasbourg 1, CNRS, UMR 7518, 12 Rue Univ, F-67000 Strasbourg, France Univ Strasbourg 1 12 Rue Univ Strasbourg France F-67000 , France
Citazione:
F. Gauer et al., "Molecular cloning of the arylalkylamine-N-acetyltransferase and daily variations of its mRNA expression in the Syrian hamster pineal gland", MOL BRAIN R, 71(1), 1999, pp. 87-95

Abstract

The arylalkylamine-N-acetyltransferase (AA-NAT) expressed in the vertebrate pineal gland catalyzes the N-acetylation of the serotonin into N-acetylserotonin and is considered to be the rate limiting enzyme of the pineal melatonin synthesis. Indeed, dramatic changes in its activity throughout the 24-h period drive the large day/night variations in plasma melatonin concentrations. Recently, AA-NAT was cloned in the rat pineal. In this species, AA-NAT mRNA variations were demonstrated to be responsible of the well known AA-NAT activity and plasma melatonin circadian fluctuations. In the Syrian hamster, the pineal melatonin secretion pattern is characterized by a late-night short-duration peak of melatonin synthesis. We investigated whether this typical pattern could be due to a late-night delayed pineal AA-NAT mRNA expression. The first part of our study was dedicated to the molecular cloning of a Syrian hamster AA-NAT cDNA. A PCR-generated clone of 1045 bp encoding the AA-NAT has been isolated and sequenced. In situ hybridization usingan AA-NAT cRNA probe revealed that the AA-NAT mRNA expression undergoes strong daily fluctuations in the Syrian hamster pineal, with undetectable level in the second half of the Light period and a dramatic increase at night. After lights off, the AA-NAT mRNA expression requires 6-7 h to reach its maximum expression. This result thus suggests that the transcription of the AA-NAT mRNA in the Syrian pineal gland determines the lag period in pineal responsiveness and melatonin synthesis to darkness. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 17:55:10