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Titolo:
In vitro elution of antibiotic from antibiotic-impregnated biodegradable calcium alginate wound dressing
Autore:
Lin, SS; Ueng, SWN; Lee, SS; Chan, EC; Chen, KT; Yang, CY; Chen, CY; Chan, YS;
Indirizzi:
Chang Gung Mem Hosp, Dept Orthopaed Surg, Kweishan, Taoyuan, Taiwan Chang Gung Mem Hosp Kweishan Taoyuan Taiwan g, Kweishan, Taoyuan, Taiwan
Titolo Testata:
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
fascicolo: 1, volume: 47, anno: 1999,
pagine: 136 - 141
Fonte:
ISI
Lingua:
ENG
Soggetto:
MTT COLORIMETRIC ASSAY; FOREIGN-BODY REACTION; HEMOSTATIC ALGINATE; PMMA BEADS; IN-VITRO; INVITRO; RELEASE; POLYMETHYLMETHACRYLATE; OSTEOMYELITIS; MANAGEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, SS Chang Gung Mem Hosp, Dept Orthopaed Surg, 5,Fu Hsing St 333, Kweishan, Taoyuan, Taiwan Chang Gung Mem Hosp 5,Fu Hsing St 333 Kweishan TaoyuanTaiwan wan
Citazione:
S.S. Lin et al., "In vitro elution of antibiotic from antibiotic-impregnated biodegradable calcium alginate wound dressing", J TRAUMA, 47(1), 1999, pp. 136-141

Abstract

Objective: The authors investigated the calcium alginate dressing as a drug-delivery system fur the treatment of various surgical infections. Methods: Cytotoxicity of the calcium alginate dressing to fibroblasts and HeLa cells was evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) colorimetric assay. The calcium alginate dressing was mixed with vancomycin, and lyophilized or not lyophilized to form two types of antibiotic dressings, The antibiotic dressings were placed in 2 mt of phosphate buffered saline (PBS) or in PBS containing 0.01% Calcium ions, and incubated at 37 degrees C. The PBS was changed daily, and the removed solutions were stored at -70 degrees C until the antibiotic concentration in each sample was determined by high performance liquid chromatography assay. Results: The results suggested that the antibiotic dressings present no obvious toxic risk to their use as a drug-delivery system. The concentration of vancomycin in each sample was well above the breakpoint sensitivity concentration (the antibiotic concentration at the transition point between bacterial killing and resistance to the antibiotic) for more than 14 days. Therelease was most marked during the first 48 hours. The concentration of calcium ions in PBS and the lyophilization of the manufacture process of antibiotic dressings prolonged the antibiotic diffusion duration. The diameter of the sample inhibition zone ranged from 10 to 11 mm, and the relative activity of vancomycin ranged from 62.88% to 92.18%. Conclusion: All antibiotic dressings released bactericidal concentrations of the antibiotics in vitro for the period of time needed to treat surgicalinfections, This study offers a convenient method to meet the specific antibiotic requirement for different patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:48:27