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Titolo:
Subunit composition of Kv1 channels in human CNS
Autore:
Coleman, SK; Newcombe, J; Pryke, J; Dolly, JO;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ,England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland Inst Neurol, London WC1N 3BG, England Inst Neurol London England WC1N 3BG nst Neurol, London WC1N 3BG, England Pfizer Cent Res, Sandwich, Kent, England Pfizer Cent Res Sandwich Kent England Cent Res, Sandwich, Kent, England
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 2, volume: 73, anno: 1999,
pagine: 849 - 858
SICI:
0022-3042(199908)73:2<849:SCOKCI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED POTASSIUM CHANNELS; HETEROMULTIMERIC K+ CHANNELS; BASIC-PROTEIN GENE; BOVINE BRAIN; RAT-BRAIN; BETA-SUBUNIT; EPISODIC ATAXIA; FUNCTIONAL-CHARACTERIZATION; MONOCLONAL-ANTIBODIES; BOTULINUM NEUROTOXIN;
Keywords:
human; CNS; potassium channel; delayed rectifier; axolemma; immunoprecipitation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Dolly, JO Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ,England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ
Citazione:
S.K. Coleman et al., "Subunit composition of Kv1 channels in human CNS", J NEUROCHEM, 73(2), 1999, pp. 849-858

Abstract

The alpha subunits of Shaker-related K+ channels (Kv1.X) show characteristic distributions in mammalian brain and restricted coassembly. Despite the functional importance of these voltage-sensitive K+ channels and involvement in a number of diseases, little progress has been achieved in decipheringthe subunit composition of the (alpha)(4)(beta)(4) oligomers occurring in human CNS. Thus, the association of alpha and beta subunits was investigated in cerebral grey and white matter and spinal cord from autopsy samples. Immunoblotting established the presence of Kv1.1, 1.2, and 1.4 in all the tissues, with varying abundance. Sequential immunoprecipitations identified the subunits coassembled. A putative tetramer of Kv1.3/1.4/1.1/1.2 was foundin grey matter. Both cerebral white matter and spinal cord contained the heterooligomers Kv1.1/1.4 and Kv1.1/1.2, similar to grey matter, but both lacked Kv1.3 and the Kv1.4/1.2 combination. An apparent Kv1.4 homooligomer was detected in all the samples, whereas only the brain tissue possessed a putative Kv1.2 homomer. In grey matter, KV beta 2.1 was coassociated with theKv1.1/1.2 combination and Kv1.2 homooligomer. In white matter, Kv beta 2.1was associated with Kv1.2 only, whereas Kv beta 1.1 coprecipitated with all the alpha subunits present. This represents the first description of Kv1 subunit complexes in the human CNS and demonstrates regional variations, indicative of functional specialisation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 19:30:27