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Titolo:
Repeated administration of dexamethasone increases phosphoinositide-specific phospholipase C activity and mRNA and protein expression of the phospholipase C beta(1) isozyme in rat brain
Autore:
Dwivedi, Y; Pandey, GN;
Indirizzi:
Univ Illinois, Inst Psychiat, Dept Psychiat, Coll Med, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 chiat, Coll Med, Chicago, IL 60612 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 2, volume: 73, anno: 1999,
pagine: 780 - 790
SICI:
0022-3042(199908)73:2<780:RAODIP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPRESSED-PATIENTS; ALPHA-SUBUNITS; KINASE-C; GLUCOCORTICOID RECEPTOR; BINDING-SITES; PLATELETS; HIPPOCAMPUS; SEROTONIN; CELLS; ADRENALECTOMY;
Keywords:
dexamethasone; rat brain; phospholipase C; immunolabeling; glucocorticoids; hypothalamic-pituitary-adrenal axis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Pandey, GN Univ Illinois, Inst Psychiat, Dept Psychiat, Coll Med, M-C 912,1601 W Taylor St, Chicago, IL 60612 USA Univ Illinois M-C 912,1601 W TaylorSt Chicago IL USA 60612 USA
Citazione:
Y. Dwivedi e G.N. Pandey, "Repeated administration of dexamethasone increases phosphoinositide-specific phospholipase C activity and mRNA and protein expression of the phospholipase C beta(1) isozyme in rat brain", J NEUROCHEM, 73(2), 1999, pp. 780-790

Abstract

Altered hypothalamic-pituitary-adrenal (HPA) function has been shown to beassociated with changes in mood and behavior. The enzyme phosphoinositide-specific phospholipase C (PI-PLC), an important component of the PI signal transduction system, plays a major role in mediating various physiological functions. In the present study, we investigated the effects of a single dose and of repeated administration (0.5 or 1.0 mg/kg for 10 days) of dexamethasone (DEX), a synthetic glucocorticoid, on PI-PLC activity and on expression of PLC isozymes (beta(1), delta(1), and gamma(1)) in rat brain. Repeated administration of DEX (1.0 mg/kg) caused a significant increase in PI-PLCactivity and in protein expression of the PLC beta(1) isozyme in both membrane and cytosol fractions of cortex and hippocampus; however, the repeatedadministration of a smaller dose of DEX (0.5 mg/kg) caused these changes only in hippocampus but not in cortex. The increase in PLC beta(1) protein was associated with an increase in its mRNA level, as measured by competitive RT-PCR. A single administration of DEX (0.5 or 1.0 mg/kg) to rats had no significant effects on PI-PLC activity or on the protein expression of PLC isozymes. These results suggest that DEX up-regulates PI-PLC in rat brain, which presumably is due to a selective increase in expression of the PLC beta(1) isozyme, and that these changes in PI-PLC may be related to HPA axis-mediated changes in mood and behavior.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:52:54