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Titolo:
Fluorescent excitation transfer immunoassay for the determination of spinosyn A in water
Autore:
Lee, M; Walt, DR; Nugent, P;
Indirizzi:
Tufts Univ, Dept Chem, Max Tishler Lab Organ Chem, Medford, MA 02155 USA Tufts Univ Medford MA USA 02155 ler Lab Organ Chem, Medford, MA 02155 USA Dow Chem Co, Midland, MI 48667 USA Dow Chem Co Midland MI USA 48667Dow Chem Co, Midland, MI 48667 USA
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 7, volume: 47, anno: 1999,
pagine: 2766 - 2770
SICI:
0021-8561(199907)47:7<2766:FETIFT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; CONTROL AGENT SPINOSAD; ULTRAVIOLET DETECTION; REAGENT DELIVERY; FLUOROIMMUNOASSAY; METABOLITES; SOIL;
Keywords:
immunoassay; fluorescent excitation transfer; spinosyn; fiber optic; water;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Walt, DR Tufts Univ, Dept Chem, Max Tishler Lab Organ Chem, Medford, MA 02155 USA Tufts Univ Medford MA USA 02155 rgan Chem, Medford, MA 02155 USA
Citazione:
M. Lee et al., "Fluorescent excitation transfer immunoassay for the determination of spinosyn A in water", J AGR FOOD, 47(7), 1999, pp. 2766-2770

Abstract

A fluorescent excitation transfer immunoassay for spinosyn A, a fermentation derived insect control agent, has been developed and applied to the analysis of tap water and wastewater effluent from manufacturing plants. Fluorescein (F) and tetramethylrhodamine (TMR) were chosen as donor and quencher,respectively, for the excitation transfer. Fluorescence quenching was observed from the binding of F-labeled antigen to TMR-labeled antibody. By employing nonlabeled antigen in a competitive immunoassay format, we reversed fluorescence quenching. The assay provides a limit of detection of 0.01 ppb and a working range of 0.05-1 ppb and allows for the rapid determination ofspinosyn A in water with recovery values ranging from 96% to 120%. With the exploitation of the small size of optical fibers, fluorescence from an assay volume of 24 mu L could be measured without special vessels.

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Documento generato il 22/01/20 alle ore 22:38:06