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Titolo:
Umbilical cord blood from preterm human fetuses is rich in committed and primitive hematopoietic progenitors with high proliferative and self-renewalcapacity
Autore:
Wyrsch, A; dalle Carbonare, V; Jansen, W; Chklovskaia, E; Nissen, C; Surbek, D; Holzgreve, W; Tichelli, A; Wodnar-Filipowicz, A;
Indirizzi:
Univ Basel Hosp, Dept Res, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 es, CH-4031 Basel, Switzerland Univ Basel Hosp, Dept Obstet & Gynecol, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 ol, CH-4031 Basel, Switzerland Univ Basel Hosp, Hematol Lab, CH-4031 Basel, Switzerland Univ Basel Hosp Basel Switzerland CH-4031 ab, CH-4031 Basel, Switzerland
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 8, volume: 27, anno: 1999,
pagine: 1338 - 1345
SICI:
0301-472X(199908)27:8<1338:UCBFPH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; STEM-CELL TRANSPLANTATION; IN-UTERO TRANSPLANTATION; BONE-MARROW CELLS; INUTERO TRANSPLANTATION; UNRELATED DONORS; APLASTIC-ANEMIA; GENE-TRANSFER; ENGRAFTMENT; VITRO;
Keywords:
hematopoietic stem cells; term cord blood; preterm cord blood; expansion; growth factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Wodnar-Filipowicz, A Lab Expt Hematol, Res Dept, Hebelstr 20, CH-4031 Basel, Switzerland Lab Expt Hematol Hebelstr 20 Basel Switzerland CH-4031
Citazione:
A. Wyrsch et al., "Umbilical cord blood from preterm human fetuses is rich in committed and primitive hematopoietic progenitors with high proliferative and self-renewalcapacity", EXP HEMATOL, 27(8), 1999, pp. 1338-1345

Abstract

Human umbilical cord blood (CB) has been recognized as a source of hematopoietic stem cells for transplantation. While hematopoietic properties of neonatal CB from full-term pregnancies have been well characterized, little is known about CB from early gestational ages. We analyzed the content and the growth properties of primitive and committed hematopoietic progenitors in preterm CB from second trimester (week 16-28; n = 17) and early third trimester (week 29-34; n = 17) in comparison with term CB (n = 18). The frequency of CD34(+) and CD34(+)CD38(-) cells was significantly higher in pretermthan in term CB (mean, 2.51% and 0.56% vs 0.88% and 0.13%; p < 0.002), Thenumber of colony forming units (CFU) in preterm CB was about twofold higher (230 +/- 6 vs 133 +/- 14/10(5) mononuclear cells; p < 0.05) and correlated with the content of CD34(+) progenitors (r = 0.73), Long-term culture initiating cells (LTC-IC) were enriched about 2.5-fold (6.7 +/- 2.9 vs 2.6 +/-1.2/10(5) cells; p < 0.05), Progenitors from preterm CB could be expanded in stroma-free liquid cultures supplemented with hematopoietic growth factors as efficiently as progenitors from term neonates, Tn short-term culturescontaining erythropoietin (Epo), interleukin (IL)-1, IL-3, and IL-6, or granulocyte- (G-) and granulocyte-macrophage colony-stimulating factor (GM-CSF) together with stem cell factor (SCF) or Flt3 ligand (FL), expansion of CFUs was six- to eightfold at week 1, In long-term cultures containing thrombopoietin (TPO) and FL, an approximately 1000-fold expansion of multilineage progenitors was observed at week 10, In summary, we show that preterm CB compared with term CB is richer in hematopoietic progenitors, and that precursors from preterm CB can be extensively expanded ex vivo. This may have implications for the development of transplantation and gene transfer strategies targeting circulating fetal stem cells. (C) 1999 International Societyfor Experimental Hematology. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:09:48