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Titolo:
Cytokines induce increased endothelin ETB receptor-mediated contraction
Autore:
Uddman, E; Moller, S; Adner, M; Edvinsson, L;
Indirizzi:
Univ Lund Hosp, Dept Internal Med, Div Expt Vasc Res, S-22185 Lund, SwedenUniv Lund Hosp Lund Sweden S-22185 v Expt Vasc Res, S-22185 Lund, Sweden
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 3, volume: 376, anno: 1999,
pagine: 223 - 232
SICI:
0014-2999(19990709)376:3<223:CIIEER>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; ORGAN-CULTURE; B RECEPTORS; SMOOTH-MUSCLE; SKIN MICROCIRCULATION; MESENTERIC-ARTERIES; ET(B) RECEPTORS; UP-REGULATION; RAT; ANTAGONISTS;
Keywords:
interleukin-1 beta; TNF-alpha (tumor necrosis factor-alpha); interleukin-2; endothelin-1; sarafotoxin 6c; IRL 1620; endothelin ETB receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Uddman, E Univ Lund Hosp, Dept Internal Med, Div Expt Vasc Res, EB Blocket,5 Van, S-22185 Lund, Sweden Univ Lund Hosp EB Blocket,5 Van Lund Sweden S-22185 und, Sweden
Citazione:
E. Uddman et al., "Cytokines induce increased endothelin ETB receptor-mediated contraction", EUR J PHARM, 376(3), 1999, pp. 223-232

Abstract

The effect of cytokines on the induction of contractile endothelin ETB receptors during organ culture was examined. Ring segments of rat superior mesenteric artery were used fresh or incubated for 24 h in Dulbecco's modifiedEagle's medium alone, or with either interleukin-1 beta, tumor necrosis factor-alpha (TNF-alpha) or interleukin-2. In fresh arterial segments there was no endothelin ETB receptor-induced contraction. After incubation, the selective endothelin ETB receptor agonist sarafotoxin 6c evoked a contractionof 22 +/- 6% relative to that induced by 60 mM K+. The endothelin ETB receptor-induced contraction was further increased to 125 +/- 25% and 157 +/- 29% by interleukin-1 beta and TNF-alpha; respectively, while interleukin-2 did not alter the endothelin ETB receptor-induced contraction. The identity of the contractile receptor was confirmed as the endothelin ETB receptor bythe use of an additional specific endothelin ETB receptor agonist, IRL 1620, and by antagonist experiments with FR 139317 and IRL 2500. The endothelin-1-induced contraction was not altered by either of the cytokines. Reversetranscriptase-polymerase chain reaction revealed increased levels of endothelin ETB mRNA, relative to endothelin ETA mRNA following organ culture, suggesting that contractile endothelin ETB receptors appear via de novo transcription. None of the cytokines changed the ratio of endothelin ETA and endothelin ETB receptor mRNA, indicating that the further increased sarafotoxin 6c-induced contraction is mediated through an enhancement of intracellular signalling mechanisms. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:18:16