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Titolo:
SIMULTANEOUS ENZYMATIC-SYNTHESIS OF GLUCONIC ACID AND SORBITOL - PRODUCTION, PURIFICATION, AND APPLICATION OF GLUCOSE-FRUCTOSE OXIDOREDUCTASE AND GLUCONOLACTONASE
Autore:
NIDETZKY B; FURLINGER M; GOLLHOFER D; HAUG I; HALTRICH D; KULBE KD;
Indirizzi:
AGR UNIV VIENNA,INST FOOD TECHNOL,DIV BIOCHEM ENGN,MUTHGASSE 18 A-1190 VIENNA AUSTRIA
Titolo Testata:
Applied biochemistry and biotechnology
, volume: 63-5, anno: 1997,
pagine: 173 - 188
SICI:
0273-2289(1997)63-5:<173:SEOGAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME REGENERATION SYSTEM; CHARGED MEMBRANE BIOREACTOR; ZYMOMONAS-MOBILIS; REACTOR;
Keywords:
GLUCOSE-FRUCTOSE OXIDOREDUCTASE; LUCONOLACTONASE; ZYMOMONAS MOBILIS; RHODOTORULA RUBRA; STABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
B. Nidetzky et al., "SIMULTANEOUS ENZYMATIC-SYNTHESIS OF GLUCONIC ACID AND SORBITOL - PRODUCTION, PURIFICATION, AND APPLICATION OF GLUCOSE-FRUCTOSE OXIDOREDUCTASE AND GLUCONOLACTONASE", Applied biochemistry and biotechnology, 63-5, 1997, pp. 173-188

Abstract

With regard to the enzymatic synthesis of sorbitol and gluconic acid,a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected forthe synthesis of GFOR and GL, respectively. Maximal enzyme productionby these organisms has been achieved at D-glucose concentrations of 200 and 150 g/L, respectively. Both GFOR and GL were purified and characterized with respect to some of their catalytic properties. GL showedstrict specificity for 1,5-(delta)-lactones and was activated by Mg2and Mn2+ ions. The potential use of soluble GFOR is limited by its inactivation during substrate conversion, and the effects of reaction temperature and pH on the ''catalytic'' stability of GFOR were evaluated. Exogenous adddition of auxiliary GL had no effect on oxidoreductase stability and did not improve productivities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:27:21