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Titolo:
A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein
Autore:
Hoss, M; Robins, P; Naven, TJP; Pappin, DJC; Sgouros, J; Lindahl, T;
Indirizzi:
Imperial Canc Res Fund, Clare Hall Labs, S Mimms EN6 3LD, Herts, England Imperial Canc Res Fund S Mimms Herts England EN6 3LD 3LD, Herts, England Lincolns Inn Fields Labs, London WC2 3PX, England Lincolns Inn Fields Labs London England WC2 3PX London WC2 3PX, England
Titolo Testata:
EMBO JOURNAL
fascicolo: 13, volume: 18, anno: 1999,
pagine: 3868 - 3875
SICI:
0261-4189(19990701)18:13<3868:AHDEEH>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASE-EXCISION-REPAIR; SCHIZOSACCHAROMYCES-POMBE RAD1(+); POLYMERASE-III HOLOENZYME; EXONUCLEASE ACTIVITY; DEOXYRIBONUCLEIC-ACID; REPLICATION; GENE; BETA; SUBUNIT; EPSILON;
Keywords:
3 ' exonuclease; mammalian DNase III; mass spectrometry protein sequencing; repair and replication fidelity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Lindahl, T Imperial Canc Res Fund, Clare Hall Labs, S Mimms EN6 3LD, Herts, England Imperial Canc Res Fund S Mimms Herts England EN6 3LD , England
Citazione:
M. Hoss et al., "A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein", EMBO J, 18(13), 1999, pp. 3868-3875

Abstract

Mammalian DNA polymerases alpha and beta lack 3' exonuclease activity and are unable to edit errors after DNA synthesis. However, editing exonucleases can be functions of separate polypeptides. We isolated a widely distributed DNA-specific 3' exonuclease from rabbit liver nuclei, sequenced tryptic peptides by mass spectrometry, and identified the corresponding human open reading frame, The protein expressed from the cloned human sequence exhibits 3' exonuclease activity. The human clone shares sequence homology with the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD protein, and weakly with the editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon. The gene maps to human chromosome 3p21.2-21.3. In a reconstituted human DNA repair system containing DNA polymerase beta and DNA ligase III-XRCC1, accurate rejoining of a 3' mismatchedbase residue at single-strand break is dependent on addition of the exonuclease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 02:36:31