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Titolo:
Increase of human spermatozoa intracellular Ca2+ concentration after fusion with prostasomes
Autore:
Palmerini, CA; Carlini, E; Nicolucci, A; Arienti, G;
Indirizzi:
Univ Perugia, Ist Biochim & Chim Med, I-06126 Perugia, Italy Univ PerugiaPerugia Italy I-06126 im & Chim Med, I-06126 Perugia, Italy Univ Perugia, Dipartimento Biol Cellulare & Mol, I-06126 Perugia, Italy Univ Perugia Perugia Italy I-06126 llulare & Mol, I-06126 Perugia, Italy
Titolo Testata:
CELL CALCIUM
fascicolo: 4, volume: 25, anno: 1999,
pagine: 291 - 296
SICI:
0143-4160(199904)25:4<291:IOHSIC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SEMINAL PLASMA; EXTRACELLULAR ORGANELLES PROSTASOMES; HUMAN-SPERM; PREVENTS SPERM; HUMAN-SEMEN; CALCIUM; PROGESTERONE; MEMBRANE; MOTILITY; PH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Arienti, G Univ Perugia, Ist Biochim & Chim Med, Via Giochetto, I-06126 Perugia, Italy Univ Perugia Via Giochetto Perugia Italy I-06126 erugia, Italy
Citazione:
C.A. Palmerini et al., "Increase of human spermatozoa intracellular Ca2+ concentration after fusion with prostasomes", CELL CALC, 25(4), 1999, pp. 291-296

Abstract

Prostasomes are membranous vesicles (150-200 nm diameter) present in humansemen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin and calcium, and some of their proteins are enzymes. Prostasomes are involved in a number of biological functions. In previous work, we discovered that prostasomes may fuse to sperm at neutral or at slightly acidic pH values. This mechanism may deliver calcium to sperm, thereby influencing sperm functions. We measured sperm [Ca2+](i) with the fura-2 AM method and found that it increased after mixing prostasomes and sperm at pH values allowing fusion (pH5-7). The increase of [Ca2+](i) was proportional to the extent of fusion as measured through the relief of R-18 self-quenching. We also examined the increase of sperm [Ca2+](i) and the extent of fusion as a function of spermto prostasome ratio and, also in this case, there was proportionality between the extent of fusion and the increase of [Ca2+](i) that reached its maximal values in about 10-20 min. However, a detectable increase of [Ca2+](i)was attained after 2 min of fusion. This would represent a new mechanism to influence sperm [Ca2+](i) besides ion-exchange systems and ATP-dependent pumps. The value of [Ca2+](i) remained elevated, unless Na+ was also present in the external medium. Therefore, the mechanism of fusion might influence deeply the physiology of sperm by producing a transient increase of [Ca2+](i).

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Documento generato il 13/07/20 alle ore 09:11:53