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Titolo:
Expression of constitutive nitric oxide synthase in rat and human gastrointestinal tract
Autore:
Fischer, H; Becker, JC; Boknik, P; Huber, V; Luss, H; Neumann, J; Schmitz, W; Domschke, W; Stachura, J; Konturek, JW;
Indirizzi:
Univ Munster, Med Klin & Poliklin B, Dept Med B, D-48129 Munster, Germany Univ Munster Munster Germany D-48129 ept Med B, D-48129 Munster, Germany Univ Munster, Inst Pharmacol & Toxicol, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 l & Toxicol, D-4400 Munster, Germany Jagiellonian Univ, Inst Pathomorphol, Krakow, Poland Jagiellonian Univ Krakow Poland Univ, Inst Pathomorphol, Krakow, Poland
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
fascicolo: 3, volume: 1450, anno: 1999,
pagine: 414 - 422
SICI:
0167-4889(19990708)1450:3<414:EOCNOS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUCOSA; PURIFICATION; FLAVOPROTEIN; LOCALIZATION; STOMACH; DAMAGE; GENE;
Keywords:
nitric oxide synthase; esophageal and gastric mobility; gastric microcirculation; mucosal protection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Fischer, H Univ Munster, Med Klin & Poliklin B, Dept Med B, Albert Schweitzer Str 33,D-48129 Munster, Germany Univ Munster Albert Schweitzer Str 33 Munster Germany D-48129
Citazione:
H. Fischer et al., "Expression of constitutive nitric oxide synthase in rat and human gastrointestinal tract", BBA-MOL CEL, 1450(3), 1999, pp. 414-422

Abstract

The aim of this study was to determine the expression of constitutive NO syntheses (ecNOS and bNOS) at the protein level in rat and human gastrointestinal tract. We established a quantitative Western blotting method for detection and quantification of ecNOS and bNOS in both species. Human gastric fundus was further analyzed by immunohistochemistry. EcNOS expression at theprotein level could be quantified in different organs of the rat gastrointestinal tract and in human gastric mucosal biopsies. Immunohistochemistry of gastric fundus revealed that immunoreactivity for ecNOS was localized mainly in the endothelium of small vessels. In rats, expression of bNOS at theprotein level was highest in esophagus. By means of immunohistochemistry of human gastric fundus, immunoreactivity was detected mainly in the plexus of Auerbach. We conclude that isoforms of constitutive nitric oxide synthase can be identified and quantified at the protein level both in rat and human gastrointestinal tract. The presence of bNOS in nerve tissue supports previous observations that NO serves as a transmitter in non-adrenergic, non-cholinergic nerves in human esophagus and stomach. The observation that ecNOS has been found mainly in endothelial cells suggests the involvement of NO in the regulation of mucosal blood flow. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:43:03