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Titolo:
Effects of the mitogen concanavalin A on pathways of thymocyte energy metabolism
Autore:
Krauss, S; Buttgereit, F; Brand, MD;
Indirizzi:
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England Humboldt Univ, Med Klin mS Rheumatol, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 Rheumatol, D-10117 Berlin, Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
fascicolo: 2, volume: 1412, anno: 1999,
pagine: 129 - 138
SICI:
0005-2728(19990630)1412:2<129:EOTMCA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTATO-TUBER MITOCHONDRIA; DOWN ELASTICITY ANALYSIS; A-STIMULATED THYMOCYTES; OXIDATIVE-PHOSPHORYLATION; PROTONMOTIVE FORCE; RESPIRATION RATE; CELLS; LYMPHOCYTES; METHYLPREDNISOLONE; CALCIUM;
Keywords:
concanavalin A; stimulation of respiration; oxidative phosphorylation; mitochondrial proton leak; regulation of energy metabolism; metabolic control analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Krauss, S Univ Cambridge, Dept Biochem, Tennis Court Rd, Cambridge CB2 1QW, England Univ Cambridge Tennis Court Rd Cambridge England CB2 1QW ngland
Citazione:
S. Krauss et al., "Effects of the mitogen concanavalin A on pathways of thymocyte energy metabolism", BBA-BIOENER, 1412(2), 1999, pp. 129-138

Abstract

The lectin concanavalin A (Con A) acts as a mitogen that preferentially activates T-ceIls. It stimulates the energy metabolism of thymocytes within seconds of exposure. We studied short-term effects ( < 30 min) of Con A on aconceptually simplified model system of rat thymocyte energy metabolism inthe concentration range of 0-2 mu g Con A per 10(7) cells, using metaboliccontrol analysis. The model system consisted of three blocks of reactions,linked by the common intermediate mitochondrial membrane potential(Delta psi(m)): the substrate oxidation reactions, which produce the linking intermediate, and the proton conductance (or leak) and ATP turnover pathways which consume Delta psi(m), Firstly, we used top-down elasticity analysis to establish which subsystems are targeted by Con A. Secondly, we quantitativelyanalysed the steady-state regulation of the system variables by Con A: howdo the subsystem fluxes respond to Con A individually and as a whole? Our results indicate that: (1) steady-state respiration and Delta psi(m) increase as Con A concentration is raised, but at higher concentrations the increase in respiration is less and Delta psi(m), falls 1 (2) Con A independently changes the kinetics of the reactions that produce and consume Delta psi(m): the Delta psi(m)-producing reactions are inhibited, and the reactions involved in ATP turnover are stimulated; and (3) the overall effects of Con A are mostly mediated by effects on ATP turnover. (C) 1999 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 02:25:22