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Titolo:
Rapid internalization and surface expression of a functional, fluorescently tagged G-protein-coupled glutamate receptor
Autore:
Doherty, AJ; Coutinho, V; Collingridge, GL; Henley, JM;
Indirizzi:
Univ Bristol, Sch Med Sci, Dept Anat, MRC Ctr Synapt Plast, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 341, anno: 1999,
parte:, 2
pagine: 415 - 422
SICI:
0264-6021(19990715)341:<415:RIASEO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MGLUR1 MUTANT MICE; BETA(2)-ADRENERGIC RECEPTOR; SYNAPTIC PLASTICITY; LIVING CELLS; KINASE-C; TRAFFICKING; VISUALIZATION; MEMBRANE; CLONING; HOMER;
Keywords:
green fluorescent protein; mGlu(1) receptor; metabotropic glutamate receptor; receptor movement;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Henley, JM Univ Bristol, Sch Med Sci, Dept Anat, MRC Ctr Synapt Plast, Univ Walk, Bristol BS8 1TD, Avon, England Univ Bristol Univ Walk Bristol Avon England BS8 1TD on, England
Citazione:
A.J. Doherty et al., "Rapid internalization and surface expression of a functional, fluorescently tagged G-protein-coupled glutamate receptor", BIOCHEM J, 341, 1999, pp. 415-422

Abstract

L-Glutamate is the principal excitatory neurotransmitter in the vertebratecentral nervous system, where it mediates many of its actions via G-protein-coupled metabotropic glutamate (mGlu) receptors. Since little is known about the dynamics of mGlu receptors at the plasma membrane, we have constructed a fusion protein comprising the mGlu receptor subtype 1 alpha (mGlu(1 alpha)) and green fluorescent protein (GFP). Using imaging of Ca2+ release from intracellular stores as a functional assay, the agonist pharmacology ofthis fluorescently tagged receptor was found to be similar to that of the wild-type receptor when expressed in HEK-293 cells. Receptor movement and function were measured simultaneously by combined imaging of Ca2+, using fura-red, and GFP fluorescence in single cells. Exposure to agonist induced a rapid loss of up to 30% of membrane-associated fluorescence, with a corresponding decrease in the functional response. Following removal of the agonist there was recovery of both the membrane fluorescence and the functional response. These data suggest that the surface expression of G-protein-coupled glutamate receptors might be rapidly regulated in response to agonist activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:21:28