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Titolo:
Hydroperoxidase activity of lipoxygenase in the presence of cyclodextrins
Autore:
Nunez-Delicado, E; Sojo, MM; Sanchez-Ferrer, A; Garcia-Carmona, F;
Indirizzi:
Univ Murcia, Fac Biol, Dept Biochem & Mol Biol A, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 hem & Mol Biol A, E-30071 Murcia, Spain
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 2, volume: 367, anno: 1999,
pagine: 274 - 280
SICI:
0003-9861(19990715)367:2<274:HAOLIT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-PEROXIDE; LINOLEIC-ACID; XENOBIOTIC METABOLISM; SOYBEAN LIPOXYGENASE; ENZYMATIC OXIDATION; CATALYZED OXIDATION; POLYPHENOL OXIDASE; MONOPHENOLASE; PURIFICATION; CHOLESTEROL;
Keywords:
lipoxygenase; hydroperoxidase; xenobiotics; diphenols; quinones; phenolic compounds; cyclodextrins; complexation constant (K-c);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Sanchez-Ferrer, A Univ Murcia, Fac Biol, Dept Biochem & Mol Biol A, CampusEspinardo, E-30071 Murcia, Spain Univ Murcia Campus Espinardo Murcia Spain E-30071 Spain
Citazione:
E. Nunez-Delicado et al., "Hydroperoxidase activity of lipoxygenase in the presence of cyclodextrins", ARCH BIOCH, 367(2), 1999, pp. 274-280

Abstract

The oxidation of xenobiotics by the hydroperoxidase activity of lipoxygenase in the presence of cyclodextrins was studied. These produced an inhibitory effect on xenobiotics oxidation, based on their degree of hydrophobicityand the charge (isoproterenol < 4-methyl-catechhol (4MC) < 4-tert-butylcatechol (TBC) < 4-tert-octylcatechol (TOC)). This inhibitory effect was due to the complexation of xenobiotics in the hydro phobic cavity of cyclodextrins. The complexation constant K-c was calculated by nonlinear regression ofthe inhibition curves obtained in the presence of cyclodextrins, and the values obtained were 400, 16,250, and 35,127 M-1 for 4MC, TBC, and TOC, respectively. The validity of these values was checked at different points of the Michaelis-Menten saturation curve, and a sigmoidal inhibition curve was obtained at the saturating concentration of the o-diphenol, TBC, with no change in the K-c value. This demonstrates the validity of the equations usedto calculate K-c for the complete range of the Michaelis-Menten equation. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 23:01:12