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Titolo:
Phagocytic and macropinocytic activity in MARCKS-deficient macrophages andfibroblasts
Autore:
Carballo, E; Pitterle, DM; Stumpo, DJ; Sperling, RT; Blackshear, PJ;
Indirizzi:
NIEHS, Off Clin Res, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 lin Res, Res Triangle Pk, NC 27709 USA NIEHS, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ansduct, Res Triangle Pk, NC 27709 USA Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 iv, Med Ctr, Dept Med, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Med Ctr, Dept Biochem, Durham, NC 27710 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 1, volume: 46, anno: 1999,
pagine: C163 - C173
SICI:
0363-6143(199907)46:1<C163:PAMAIM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; RECEPTOR-MEDIATED PHAGOCYTOSIS; COLONY-STIMULATING FACTOR; MARROW-DERIVED MACROPHAGES; ABNORMAL BRAIN-DEVELOPMENT; NEURAL-TUBE DEFECTS; SUBSTRATE MARCKS; TYROSINE PHOSPHORYLATION; MEMBRANE ASSOCIATION; PHORBOL ESTERS;
Keywords:
phagocytosis; macropinocytosis; myristoylated; alanine-rich; C kinase substrate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Blackshear, PJ NIEHS, Off Clin Res, A2-05,111 Alexander Dr, Res Triangle Pk, NC 27709 USA NIEHS A2-05,111 Alexander Dr Res Triangle Pk NC USA 27709 A
Citazione:
E. Carballo et al., "Phagocytic and macropinocytic activity in MARCKS-deficient macrophages andfibroblasts", AM J P-CELL, 46(1), 1999, pp. C163-C173

Abstract

Macrophages express high levels of the myristoylated, alanine-rich, C kinase substrate (MARCKS), an actin cross-linking protein. To investigate a possible role of MARCKS in macrophage function, fetal liver-derived macrophages were generated from wild-type and MARCKS knockout mouse embryos. No differences between the wild-type and MARCKS-deficient macrophages with respect to morphology (Wright's stain) or actin distribution (staining with rhodamine-phalloidin, under basal conditions or after treatment with phorbol esters, lipopolysaccharide, or both) were observed. We then evaluated phagocytosis mediated by different receptors: Fc receptors tested with IgG-coated sheep red blood cells, complement C3b receptors tested with C3b-coated yeast, mannose receptors tested with unopsonized zymosan, and nonspecific phagocytosis tested with latex beads. We also studied fluid phase endocytosis in macrophages and mouse embryo fibroblasts by using FITC-dextran to quantitate this process. In most cases, there were no differences between the cells derived from wild-type and MARCKS-deficient mice. However, a minor but significant and reproducible difference in rates of zymosan phagocytosis at 45-60min was observed, with lower rates of phagocytosis in the MARCKS-deficientcells. Our data indicate that MARCKS deficiency may lead to slightly decreased rates of zymosan phagocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 06:28:51