Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A human DAZ transgene confers partial rescue of the mouse Dazl null phenotype
Autore:
Slee, R; Grimes, B; Speed, RM; Taggart, M; Maguire, SM; Ross, A; McGill, NI; Saunders, PTK; Cooke, HJ;
Indirizzi:
Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland Western Gen Hosp Edinburgh Midlothian Scotland EH4 2XU dlothian, Scotland MRC, Ctr Reprod Biol, Reprod Biol Unit, Edinburgh EH3 9EW, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9EW h EH3 9EW, Midlothian, Scotland
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 96, anno: 1999,
pagine: 8040 - 8045
SICI:
0027-8424(19990706)96:14<8040:AHDTCP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN Y-CHROMOSOME; RNA-BINDING PROTEIN; MALE-STERILITY GENE; AZOOSPERMIC MALES; MALE-INFERTILITY; AUTOSOMAL GENE; GERM-CELLS; LONG ARM; HOMOLOG; SPERMATOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Cooke, HJ Western Gen Hosp, MRC, Human Genet Unit, Crewe Rd, Edinburgh EH42XU, Midlothian, Scotland Western Gen Hosp Crewe Rd Edinburgh Midlothian Scotland EH4 2XU
Citazione:
R. Slee et al., "A human DAZ transgene confers partial rescue of the mouse Dazl null phenotype", P NAS US, 96(14), 1999, pp. 8040-8045

Abstract

In a subset of infertile men, a spectrum of spermatogenic defects ranging from a complete absence of germ cells (sertoli cell only) to oligozoospermia is associated with microdeletions, of the DAZ (deleted in azoospermia) gene cluster on human distal Yq. DAZ encodes a testis-specific protein with RNA-binding potential recently derived from a single-copy gene DAZL1 (DAZ-like) on chromosome 3. Y chromosomal DAZ homologues are confined to humans and higher primates. It remains unclear which function unique to higher primate spermatogenesis DAZ may serve, and the functional status of the gene recently has been questioned. To assess the extent of functional conservation we have tested the capacity of-a humanDAZ gene contained in a 225-kb yeast artificial chromosome to complement the sterile phenotype of the Dad null mouse (Dazl(-/-)), which is characterized by severe germ-cell depletion and meiotic failure. Although Dazl(-/-) mice remained infertile when the DAZ transgene was introduced, histological examination revealed a partial and variable rescue of the mutant phenotype, manifest as a pronounced increase in the germ cell population of the seminiferous tubules and survival to the pachytene stage of meiosis. As well as constituting definitive proof of the spermatogenic role of the DAZ gene product, these findings confirm the high degree of functional conservation between the DAZ and DAZL1 genes, suggesting they may constitute a single target for contraceptive intervention and raising the possibility of therapeutic up-regulation of the DAZL1 gene in infertile men.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 20:38:06