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Titolo:
Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression
Autore:
van Hengel, J; Vanhoenacker, P; Staes, K; van Roy, F;
Indirizzi:
State Univ Ghent, Flanders Interuniv Inst Biotechnol, Dept Mol Biol, Mol Cell Biol Unit, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 l Biol Unit, B-9000 Ghent, Belgium State Univ Ghent, Flanders Interuniv Inst Biotechnol, Dept Mol Biol, Unit Eukaryot Gene Express & Signal Transduct, B-9000 Ghent, Belgium State Univ Ghent Ghent Belgium B-9000 l Transduct, B-9000 Ghent, Belgium
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 96, anno: 1999,
pagine: 7980 - 7985
SICI:
0027-8424(19990706)96:14<7980:NLOTPA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR LEF-1; MOLECULES ALPHA-CATENINS; HUMAN COLORECTAL-CANCER; SEGMENT POLARITY GENE; BETA-CATENIN; TYROSINE PHOSPHORYLATION; CELL-ADHESION; DESMOSOMAL PLAQUE; EPITHELIAL-CELLS; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: van Roy, F State Univ Ghent, Flanders Interuniv Inst Biotechnol, Dept Mol Biol, Mol Cell Biol Unit, Ledeganckstr 35, B-9000 Ghent, Belgium State UnivGhent Ledeganckstr 35 Ghent Belgium B-9000 Belgium
Citazione:
J. van Hengel et al., "Nuclear localization of the p120(ctn) Armadillo-like catenin is counteracted by a nuclear export signal and by E-cadherin expression", P NAS US, 96(14), 1999, pp. 7980-7985

Abstract

The Armadillo protein p120(ctn) associates with the cytoplasmic domain of cadherins and accumulates at cell-cell junctions. Particular Armadillo proteins such as beta-catenin and plakophilins show a partly nuclear location, suggesting gene-regulatory activities. For different human E-cadherin-negative carcinoma cancer cell lines we found expression of endogenous p120(ctn)in the nucleus. Expression of E-cadherin directed p120(ctn) out of the nucleus. Previously, we reported that the human p120(ctn) gene might encode upto 32 protein isoforms as products of alternative splicing. Overexpressionof p120(ctn) isoforms B in various cell lines resulted in cytoplasmic immunopositivity but never in nuclear staining. In contrast, upon-expression ofp120(ctn) cDNAs lacking exon B, the isoforms were detectable within both nuclei and cytoplasm. A putative nuclear export signal (NES) with a characteristic leucine-rich motif is encoded by exon B. This sequence element was shown to be required for nuclear export and to function autonomously when fused to a carrier protein and microinjected into cell nuclei. Moreover, the NES function of endogenously or exogenously expressed p120(ctn) isoforms B was sensitive to the nuclear export inhibitor leptomycin B. Expression of exogenous E-cadherin down-regulated nuclear p120(ctn) whereas activation of protein kinase C increased the level of nuclear p120(ctn). These results reveal molecular mechanisms controlling the subcellular distribution of p120(ctn).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:12:52