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Titolo:
Expression of the auxin-inducible GH3 Promoter GUS fusion gene as a usefulmolecular marker for auxin physiology
Autore:
Li, Y; Wu, YH; Hagen, G; Guilfoyle, T;
Indirizzi:
Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA Univ Connecticut Storrs CT USA 06269 Dept Plant Sci, Storrs, CT 06269 USA Univ Missouri, Dept Biochem, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Biochem, Columbia, MO 65211 USA
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 7, volume: 40, anno: 1999,
pagine: 675 - 682
SICI:
0032-0781(199907)40:7<675:EOTAGP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOBACCO MESOPHYLL PROTOPLASTS; REGULATED GENE; PLANT DEVELOPMENT; ARABIDOPSIS-THALIANA; TRANSGENIC TOBACCO; SOYBEAN HYPOCOTYL; GROWTH-SUBSTANCES; RESPONSE ELEMENTS; RAPID INDUCTION; MESSENGER-RNAS;
Keywords:
auxin; GH3 gene; gene expression; molecular marker;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Li, Y Univ Connecticut, Dept Plant Sci, U-67, Storrs, CT 06269 USA Univ Connecticut U-67 Storrs CT USA 06269 U-67, Storrs, CT 06269 USA
Citazione:
Y. Li et al., "Expression of the auxin-inducible GH3 Promoter GUS fusion gene as a usefulmolecular marker for auxin physiology", PLANT CEL P, 40(7), 1999, pp. 675-682

Abstract

Expression of the GH3 promoter/GUS reporter (GH3/ GUS) gene in transgenic tobacco is silent in most vegetative tissues and organs, but can be specifically induced by auxin in all cell types throughout the plant, indicating that auxin is a limiting factor for activation of the GH3/GUS gene. In the absence of exogenous auxin, expression of the GH3/ GUS gene either correlates with physiological events which are presumably mediated by auxin or occurs in cells where relatively high levels of endogenous auxin are expected. For example, the GH3/GUS gene is expressed in dormant lateral buds, tips of adventitious roots, and stem parenchyma cells where adventitious roots are initiating. The GH3/GUS gene is activated at the cut surface nearest the tip of a leaf from which a sl:rip of tissue has been excised, and in the basal end of an excised shoot segment. Also, the GH3/GUS gene is expressed predominantly in the lower side of a gravistimulated shoot and in the dark sideof a unilateral light-stimulated loot. Furthermore, the expression patternof the GH3/GUS gene is similar to the distribution pattern of C-14-IAA that is applied at the apical end of the gravistimulated shoot. Thus, the results reported here provide additional support for a crucial role of auxin inshoot gravi- and photo-tropisms and adventitious root formation. Furthermore, the data presented in this report also indicate that expression of the GH3/GUS gene is an excellent molecular marker for studying auxin transport and for monitoring changes in either auxin concentration or cellular sensitivity to auxin in planta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:24:09