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Titolo:
The fidelity of template-directed oligonucleotide ligation and the inevitability of polymerase function
Autore:
James, KD; Ellington, AD;
Indirizzi:
Indiana Univ, Dept Chem, Bloomington, IN 47405 USA Indiana Univ Bloomington IN USA 47405 ept Chem, Bloomington, IN 47405 USA Univ Texas, Inst Cellular & Mol Biol A4800, Austin, TX 78712 USA Univ Texas Austin TX USA 78712 lar & Mol Biol A4800, Austin, TX 78712 USA
Titolo Testata:
ORIGINS OF LIFE AND EVOLUTION OF THE BIOSPHERE
fascicolo: 4, volume: 29, anno: 1999,
pagine: 375 - 390
SICI:
0169-6149(199908)29:4<375:TFOTOL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-REPLICATING HEXADEOXYNUCLEOTIDE; PEPTIDE NUCLEIC-ACIDS; RANDOM RNA SEQUENCES; TETRAHYMENA RIBOZYME; INFORMATION-TRANSFER; WORLD; OLIGOMERS; ORIGIN; POOL; LIFE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: James, KD Indiana Univ, Dept Chem, Bloomington, IN 47405 USA Indiana UnivBloomington IN USA 47405 Bloomington, IN 47405 USA
Citazione:
K.D. James e A.D. Ellington, "The fidelity of template-directed oligonucleotide ligation and the inevitability of polymerase function", ORIGIN LIFE, 29(4), 1999, pp. 375-390

Abstract

The first living systems may have employed template-directed oligonucleotide ligation for replication. The utility of oligonucleotide ligation as a mechanism for the origin and evolution of life is in part dependent on its fidelity. We have devised a method for evaluating ligation fidelity in whichligation substrates are selected from random sequence libraries. The fidelities of chemical and enzymatic ligation are compared under a variety of conditions. While reaction conditions can be found that promote high fidelitycopying, departure from these conditions leads to error-prone copying. In particular, ligation reactions with shorter oligonucleotide substrates are less efficient but more faithful. These results support a model for originsin which there was selective pressure for template-directed oligonucleotide ligation to be gradually supplanted by mononucleotide polymerization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 14:07:02