Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway
Autore:
Chaturvedi, P; Eng, WK; Zhu, Y; Mattern, MR; Mishra, R; Hurle, MR; Zhang, XL; Annan, RS; Lu, Q; Faucette, LF; Scott, GF; Li, XT; Carr, SA; Johnson, RK; Winkler, JD; Zhou, BBS;
Indirizzi:
SmithKline Beecham Pharmaceut, Dept Oncol Res, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Mol Biol, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Bioinformat, King Of Prussia, PA 19406USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 a, PA 19406USA SmithKline Beecham Pharmaceut, Dept Phys & Struct Chem, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Gene Express Sci, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA SmithKline Beecham Pharmaceut, Dept Prot Biochem, King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut King Of Prussia PA USA 19406 , PA 19406 USA
Titolo Testata:
ONCOGENE
fascicolo: 28, volume: 18, anno: 1999,
pagine: 4047 - 4054
SICI:
0950-9232(19990715)18:28<4047:MCIADE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATAXIA-TELANGIECTASIA; PROTEIN-KINASE; FISSION YEAST; S-PHASE; SCHIZOSACCHAROMYCES-POMBE; REPLICATION; GENE; PHOSPHORYLATION; TRANSCRIPTION; CDS1;
Keywords:
Chk2; ATM; cdc25C; gamma-radiation; hydroxyurea; topotecan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Zhou, BBS SmithKline Beecham Pharmaceut, Dept Oncol Res, 709 Swedeland Rd,King Of Prussia, PA 19406 USA SmithKline Beecham Pharmaceut 709 Swedeland Rd King Of Prussia PA USA 19406
Citazione:
P. Chaturvedi et al., "Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway", ONCOGENE, 18(28), 1999, pp. 4047-4054

Abstract

In response to DNA damage and replication blocks, cells activate pathways that arrest the cell cycle and induce the transcription of genes that facilitate repair. In mammals, ATM (ataxia telangiectasia mutated) kinase together with other checkpoint kinases are important components in this response. We have cloned the rat and human homologs of Saccharomyces cerevisiae Rad 53 and Schizosaccharomyces pombe Cds1, called checkpoint kinase 2 (chk2). Complementation studies suggest that Chk2 can partially replace the functionof the defective checkpoint kinase in the Cds1 deficient yeast strain. Chk2 was phosphorylated and activated in response to DNA damage in an ATM dependent manner. Its activation in response to replication blocks by hydroxyurea (HU) treatment, however, was independent of ATM, Using mass spectrometry, me found that, similar to Chk1, Chk2 can phosphorylate serine 216 in Cdc25C, a site known to be involved in negative regulation of Cdc25C, These results suggest that Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway. Activation of Chk2 might not only delay mitotic entry, but also increase the capacity of cultured cells to survive after treatment with gamma-radiation or with the topoisomerase-I inhibitor topotecan.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:19:18