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Titolo:
Incorporation of metabolite prior knowledge for data analysis: biochemicalimplications of dynamic P-31 NMR ex vivo pig liver studies
Autore:
Changani, KK; Ala-Korpela, M; Fuller, BJ; Mierisova, S; Bryant, DJ; Taylor-Robinson, SD; Davidson, BR; Bell, JD;
Indirizzi:
Royal Free Hosp & Med Sch, Dept Surg, London NW3 2QG, England Royal Free Hosp & Med Sch London England NW3 2QG London NW3 2QG, England Royal Free Hosp & Med Sch, Liver Transplant Grp, London NW3 2QG, England Royal Free Hosp & Med Sch London England NW3 2QG London NW3 2QG, England Hammersmith Hosp, Royal Postgrad Med Sch, Robert Steiner NMR Unit, London W12 0NN, England Hammersmith Hosp London England W12 0NN MR Unit, London W12 0NN, England Hammersmith Hosp, Royal Postgrad Med Sch, Div Gastroenterol, London W12 0NN, England Hammersmith Hosp London England W12 0NN enterol, London W12 0NN, England Univ Kuopio, AI Virtanen Inst Mol Sci, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 Mol Sci, FIN-70211 Kuopio, Finland
Titolo Testata:
NMR IN BIOMEDICINE
fascicolo: 4, volume: 12, anno: 1999,
pagine: 197 - 204
SICI:
0952-3480(199906)12:4<197:IOMPKF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR MAGNETIC-RESONANCE; HUMAN BLOOD-PLASMA; MRS IN-VIVO; ABSOLUTE CONCENTRATIONS; H-1-NMR SPECTRA; HUMAN TISSUE; TIME-DOMAIN; QUANTIFICATION; SPECTROSCOPY; SIGNALS;
Keywords:
semi-automated; analysis; P-31 NMR; liver; metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Changani, KK Hammersmith Hosp, Imperial Coll, Sch Med, NMR Unit, Du Cane Rd, London W120NN, England Hammersmith Hosp Du Cane Rd London England W12 0NN , England
Citazione:
K.K. Changani et al., "Incorporation of metabolite prior knowledge for data analysis: biochemicalimplications of dynamic P-31 NMR ex vivo pig liver studies", NMR BIOMED, 12(4), 1999, pp. 197-204

Abstract

A semi-automated, metabolite prior-knowledge-based, lineshape fitting analysis has been developed to assess the dynamic biochemical changes found in ex vivo P-31 NMR pig liver preservation studies. Due to the inherent experimental limitations of the ex vivo study and the complexity of the compositephosphorus resonances, metabolite information obtained in vitro was incorporated into the ex vivo analysis. This approach has allowed complete metabolite analysis (phosphomonoesters, inorganic phosphate, phosphodiesters and nucleotide triphosphates) in over 2000 spectra in a fraction of the time compared with more conventional analysis methods. The developed analysis willenable complete and rapid assessment of the biochemical changes in ongoingcold preservation studies of the pig liver which will result in thousands of ex vivo P-31 NMR spectra. It is also envisaged that comparative studies on human donor livers will be carried out, in which this type of analysis would be the method of choice. Moreover, this kind of analysis approach could be advantageous in many complex in vivo NMR spectroscopy applications. Copyright (C) 1999 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:30:36