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Titolo:
Development of scarring and renal failure in a rat model of crescentic glomerulonephritis
Autore:
Tam, FWK; Smith, J; Morel, D; Karkar, AM; Thompson, EM; Cook, HT; Pusey, CD;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Sch Med, Div Med,Renal Sect, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Sch Med, Dept Histopathol, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 7, volume: 14, anno: 1999,
pagine: 1658 - 1666
SICI:
0931-0509(199907)14:7<1658:DOSARF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEPHROTOXIC SERUM NEPHRITIS; NITRIC-OXIDE PRODUCTION; CD8 POSITIVE CELLS; GLOMERULAR INJURY; WKY RATS; MACROPHAGE; LYMPHOCYTE;
Keywords:
crescentic; glomerular basement membrane; glomerulonephritis; nephrotoxic nephritis; Wistar Kyoto rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Tam, FWK Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Sch Med, Div Med,Renal Sect, Du Cane Rd, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med Du Cane Rd London England W12 0NN
Citazione:
F.W.K. Tam et al., "Development of scarring and renal failure in a rat model of crescentic glomerulonephritis", NEPH DIAL T, 14(7), 1999, pp. 1658-1666

Abstract

Background. The aim of this study was to develop and characterize a rat model of crescentic glomerulonephritis which progresses to glomerulosclerosisand renal failure. Methods. Glomerulonephritis was induced in Wistar Kyoto rats by a single injection of rabbit antiglomerular basement membrane antiserum. Albuminuria and serum creatinine were monitored. Kidneys were examined, from 2.5 h to 44 days, using light-microscopy and immunohistochemistry. To characterize the glomerular inflammatory infiltrate, glomeruli were digested to single cells and analysed by fluorescence-activated cell sorter (FACS) and by immunohistochemistry on cytospins. Results. Rats developed albuminuria by 4 days and increased serum creatinine by day 18. Histology showed glomerular fibrinoid necrosis by day 4 and cellular crescents in a mean of 63% of glomeruli by day 11. By 6 weeks, ratshad developed renal failure (mean creatinine > 300 mu mol/l) with 94% of the glomeruli showing glomerulosclerosis. The kidneys were also affected by severe interstitial nephritis and tubular loss. The glomeruli were infiltrated by monocytes/ macrophages (ED1+) and CD8+ (OX8+) cells. FACS analysis showed that CD8+ cells did not express T-cell markers (CD3, TCR alpha beta or TCR gamma delta) or the NK-cell marker (NKR-P1). FACS analysis of peripheral blood mononuclear cells demonstrated a population of monocytes reactivewith OX8, and double-labelling of cytospin preparations of glomerular digests showed that a proportion of the CD8+ cells were a subset of ED1+ monocyte/macrophages. Conclusions. We have characterized a reproducible model of crescentic glomerulonephritis which rapidly progresses to chronic renal failure with glomerulosclerosis and tubulo-interstitial scarring. This model will be useful for testing new therapeutic approaches in crescentic glomerulonephritis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:05:11