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Titolo:
Peroxyacetyl nitrate-induced apoptosis through generation of reactive oxygen species in HL-60 cells
Autore:
Liu, GY; Chen, KJ; Lin-Shiau, SY; Lin, JK;
Indirizzi:
Natl Taiwan Univ, Coll Med, Inst Biochem, Sect 1, Taipei, Taiwan Natl Taiwan Univ Taipei Taiwan ed, Inst Biochem, Sect 1, Taipei, Taiwan Natl Taiwan Univ, Coll Med, Inst Toxicol, Taipei, Taiwan Natl Taiwan UnivTaipei Taiwan , Coll Med, Inst Toxicol, Taipei, Taiwan
Titolo Testata:
MOLECULAR CARCINOGENESIS
fascicolo: 3, volume: 25, anno: 1999,
pagine: 196 - 206
SICI:
0899-1987(199907)25:3<196:PNATGO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEROXYNITRITE-INDUCED APOPTOSIS; NITRIC-OXIDE; ENDOTHELIAL-CELLS; GAS-PHASE; PAN; INDUCTION; PRODUCTS; KINASE; SUPEROXIDE; ACTIVATION;
Keywords:
peroxynitrite; peroxyacetyl nitrate; air pollutant; apoptosis; reactive oxygen species; SAPK JNK; reactive nitrogen species; tyrosine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, JK Natl Taiwan Univ, Coll Med, Inst Biochem, Sect 1, 1,Jen Ai Rd, Taipei, Taiwan Natl Taiwan Univ 1,Jen Ai Rd Taipei Taiwan Ai Rd, Taipei, Taiwan
Citazione:
G.Y. Liu et al., "Peroxyacetyl nitrate-induced apoptosis through generation of reactive oxygen species in HL-60 cells", MOL CARCINO, 25(3), 1999, pp. 196-206

Abstract

Peroxyacetyl nitrate (PAN), an ubiquitous air pollutant, induced apoptosisin human leukemia HL-60, human chronic myelogenous leukemia K-562, and mouse monocyte-macrophage RAW 264.7 cell lines. In the HL 60 cells, characteristic apoptosis morphology could be observed 4h after the cells were treatedwith 50 mu M PAN. Exposure of HL-60 cells to increasing concentrations of PAN (from 1 mu M to 100 mu M) confirmed the concentration dependence of apoptosis as evidenced by DNA fragmentation in HL-60 cells, chromatin condensation by acridine-orange staining, and the appearance of the DNA apoptotic peak in flow cytometry. During apoptosis in HL-60 cells, 3-nitrotyrosine and3,5-dinitrotyrosine were detected by high-performance liquid chromatography and liquid chromatography-mass spectrometry-mass spectrometry. We hypothesized that PAN might induce cell death in human leukemia cells by releasingperoxynitrite and other reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. Moreover, exogenous superoxide dismutase promoted PAN-induced apoptosis, and in contrast, a combination of superoxide dismutase and catalase suppressed this apoptosis. We also hypothesize that the generation of ROS during PAN-induced apoptosis in HL-60 cells could activate stress-activated protein kinase/jun N-terminal kinase activity. The formation of H2O2 produced from the dismutation of PAN-elicited superoxide anion contributed to the apoptotic mechanism in HL-60 cells through ROS pathways. These findings suggested that induction of apoptosis by the air pollutant PAN might occur as a result of the release of ROS. (C) 1999 Wiley-Liss, inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 09:51:49