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Titolo:
Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain
Autore:
Soltysik-Espanola, M; Rogers, RA; Jiang, SX; Kim, TA; Gaedigk, R; White, RA; Avraham, H; Avraham, S;
Indirizzi:
Harvard Inst Med, Beth Israel Deaconess Med Ctr, Div Expt Med, Boston, MA 02115 USA Harvard Inst Med Boston MA USA 02115 , Div Expt Med, Boston, MA 02115 USA Harvard Univ, Sch Publ Hlth, Biomed Imaging Lab, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Biomed Imaging Lab, Boston, MA 02115 USA Univ Missouri, Sch Med, Childrens Mercy Hosp, Sect Med Genet & Mol Med, Kansas City, MO 64108 USA Univ Missouri Kansas City MO USA 64108 Mol Med, Kansas City, MO 64108 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 7, volume: 10, anno: 1999,
pagine: 2361 - 2375
SICI:
1059-1524(199907)10:7<2361:COMANA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERACTION MOTIF; DROSOPHILA KELCH; GENE-EXPRESSION; BTB/POZ DOMAIN; BTB DOMAIN; IN-VIVO; CYTOSKELETON; SEQUENCE; ENCODES; COMMON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Avraham, S Harvard Inst Med, Beth Israel Deaconess Med Ctr, Div Expt Med, Boston, MA 02115 USA Harvard Inst Med Boston MA USA 02115 Med, Boston, MA 02115 USA
Citazione:
M. Soltysik-Espanola et al., "Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain", MOL BIOL CE, 10(7), 1999, pp. 2361-2375

Abstract

The cytoskeleton plays an important role in neuronal morphogenesis. We have identified and characterized a novel actin-binding protein, termed Mayven, predominantly expressed in brain. Mayven contains a BTB (broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger) domain-like structure inthe predicted N terminus and "kelch repeats" in the predicted C-terminal domain. Mayven shares 63% identity (77% similarity) with the Drosophila ringcanal ("kelch") protein. Somatic cell-hybrid analysis indicated that the human Mayven gene is located on chromosome 4q21.2, whereas the murine homolog gene is located on chromosome 8. The BTB/POZ domain of Mayven can self-dimerize in vitro, which might be important for its interaction with other BTB/POZ-containing proteins. Confocal microscopic studies of endogenous Mayven protein revealed a highly dynamic localization pattern of the protein. InU373-MG astrocytoma/ glioblastoma cells, Mayven colocalized with actin filaments in stress fibers and-in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, Mayven is highly expressed in the cell body and in neurite processes. Binding assays and far Western blotting analysis demonstrated association of Mayven with actin. This association is mediated through the "kelch repeats" within the C terminus of Mayven. Depolarization of primary hippocampal neurons with KCl enhanced the association of Mayven with actin. This increased association resulted in dynamic changes in Mayven distribution from uniform to punctate localization along neuronal processes. These results suggest that Mayven functions as an actin-binding protein that may be translocated along axonal processes and might be involved in the dynamic organization of the actin cytoskeleton in brain cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 12:38:57