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Titolo:
Effects of culture conditions and exposure to catabolic stimulators (IL-1 and retinoic acid) on the expression of matrix metalloproteinases (MMPs) and disintegrin metalloproteinases (ADAMs) by articular cartilage chondrocytes
Autore:
Flannery, CR; Little, CB; Caterson, B; Hughes, CE;
Indirizzi:
Univ Wales Coll Cardiff, Cardiff Sch Biosci, Connect Tissue Biol Labs, Cardiff CF1 3US, S Glam, Wales Univ Wales Coll Cardiff Cardiff S Glam Wales CF1 3US 1 3US, S Glam, Wales
Titolo Testata:
MATRIX BIOLOGY
fascicolo: 3, volume: 18, anno: 1999,
pagine: 225 - 237
SICI:
0945-053X(199906)18:3<225:EOCCAE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-LINKED METALLOPROTEINASE; POLYMERASE CHAIN-REACTION; NECROSIS-FACTOR-ALPHA; HEMOPEXIN-LIKE DOMAIN; INTERGLOBULAR DOMAIN; TNF RECEPTOR; MOLECULAR-CLONING; SEQUENCE-ANALYSIS; CLEAVAGE SITE; AGGRECAN;
Keywords:
ADAM (A Disintegrin And Metalloproteinase); cartilage; chondrocyte; matrix metalloproteinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Flannery, CR Univ Wales Coll Cardiff, Cardiff Sch Biosci, Connect Tissue Biol Labs, Museum Ave, Cardiff CF1 3US, S Glam, Wales Univ Wales Coll Cardiff Museum Ave Cardiff S Glam Wales CF1 3US
Citazione:
C.R. Flannery et al., "Effects of culture conditions and exposure to catabolic stimulators (IL-1 and retinoic acid) on the expression of matrix metalloproteinases (MMPs) and disintegrin metalloproteinases (ADAMs) by articular cartilage chondrocytes", MATRIX BIOL, 18(3), 1999, pp. 225-237

Abstract

The chondrocytes of articular cartilage synthesize a number of proteinaseswhich are capable of degrading the component molecules of this specializedextracellular matrix. The use of class-specific proteinase inhibitors indicates that major activities responsible for catabolism of proteoglycan (aggrecan) and collagen are attributable to zinc-dependent metalloproteinases. In this study, we have compared the mRNA expression profiles of two matrix metalloproteinases (MMP-3 and MMP-13) and five disintegrin-metalloproteinases (ADAM-10, ADAM-9, ADAM-15, TNF-a-converting enzyme and decysin) by chondrocytes (human, porcine and bovine) from fresh cartilage and in cartilage explant cultures and isolated cells cultured in monolayer or in agarose gels. Such cultures were maintained in the presence or absence of interleukin-1(IL-1) or all-trans-retinoic acid, two agents which promote cartilage matrix degradation in vitro. Whereas transcripts for all metalloproteinases examined were detected in chondrocytes from human osteoarthritic cartilage in monolayer cultures, mRNAs for ADAM-15 and decysin were not present in freshosteoarthritic human cartilage or explant cultures. Similarly, expression of porcine and bovine metalloproteinase mRNAs varied with different cultureconditions. Novel cDNA sequences obtained for porcine and bovine MMP-3 andMMP-13, porcine ADAM-10, porcine and bovine ADAM-9 and porcine TACE confirmed expression of mRNAs for these molecules by articular chondrocytes, Quantitative RT-PCR analysis was used to determine the effects of IL-1 and retinoic acid on metalloproteinase mRNA levels in human chondrocytes cultured in monolayer and in porcine chondrocytes cultured in agarose. For the MMPs, IL-1 treatment resulted in an approximately two to threefold increase in human and porcine MMP-3 and MMP-13 mRNAs, while retinoic acid treatment caused a statistically significant increase in human MMP-3 mRNA levels, but no significant change in transcript levels for porcine MMP-3 nor human or porcine MMP-13. The mRNA levels for ADAM-15 were elevated in hum;ln monolayer chondrocytes exposed to IL-1 or retinoic acid, while transcripts levels for TNF-alpha converting enzyme were increased in response to retinoic acid. In contrast, ADAM-9 mRNA levels were decreased in human monolayer chondrocytesexposed to IL-1 or retinoic acid. The results demonstrate that chondrocytemetalloproteinase expression can vary dependent on cell environment in situ and in vitro, and provide new information on chondrocyte MMP and ADAM gene expression following cytokine (IL-1) or retinoid stimulation. (C) 1999 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:16:08