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Titolo:
Differences in brain gene expression between sleep and waking as revealed by mRNA differential display and cDNA microarray technology
Autore:
Cirelli, C; Tononi, G;
Indirizzi:
Inst Neurosci, San Diego, CA 92121 USA Inst Neurosci San Diego CA USA 92121 st Neurosci, San Diego, CA 92121 USA
Titolo Testata:
JOURNAL OF SLEEP RESEARCH
, volume: 8, anno: 1999, supplemento:, 1
pagine: 44 - 52
SICI:
0962-1105(199906)8:<44:DIBGEB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-FOS EXPRESSION; CEREBRAL PROTEIN-SYNTHESIS; HORMONE MESSENGER-RNA; IMMEDIATE-EARLY GENES; SLOW-WAVE SLEEP; RAT-BRAIN; LOCUS-COERULEUS; DEPRIVATION INCREASES; CYCLE; MODULATION;
Keywords:
cerebral cortex; cDNA microarray; immediate early genes; mitochondria; mRNA differential display; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Cirelli, C Inst Neurosci, 10640 John Jay Hopkins Dr, San Diego, CA 92121 USA Inst Neurosci 10640 John Jay Hopkins Dr San Diego CA USA 92121
Citazione:
C. Cirelli e G. Tononi, "Differences in brain gene expression between sleep and waking as revealed by mRNA differential display and cDNA microarray technology", J SLEEP RES, 8, 1999, pp. 44-52

Abstract

The consequences of sleep and sleep deprivation at the molecular level arelargely unexplored. Knowledge of such molecular events is essential to understand the restorative processes occurring during sleep as well as the cellular mechanisms of sleep regulation. Here we review the available data about changes in neural gene expression across different behavioural states using candidate gene approaches such as in situ hybridization and immunocytochemistry. We then describe new techniques for systematic screening of gene expression in the brain, such as subtractive hybridization, mRNA differential display, and cDNA microarray technology, outlining advantages and disadvantages of these methods. Finally, we summarize our initial results of a systematic screening of gene expression in the rat brain across behavioural states using mRNA differential display and cDNA microarray technology. The expression pattern of approximate to 7000 genes was analysed in the cerebralcortex of rats after 3 h of spontaneous sleep, 3 h of spontaneous waking, or 3 h of sleep deprivation. While the majority of transcripts were expressed at the same level among these three conditions, 14 mRNAs were modulated by sleep and waking. Six transcripts, four more expressed in waking and twomore expressed in sleep, corresponded to novel genes. The eight known transcripts were all expressed at higher levels in waking than in sleep and included transcription factors and mitochondrial genes. A possible role for these known transcripts in mediating neural plasticity during waking is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:48:26