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Titolo:
Semi-automatic liquid chromatographic analysis of pamidronate in urine after derivatization with 1-naphthylisothiocyanate
Autore:
Sparidans, RW; den Hartigh, J; Cremers, S; Beijnen, JH; Vermeij, P;
Indirizzi:
Leiden Univ, Med Ctr, Dept Clin Pharm & Toxicol, NL-2300 RC Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RC NL-2300 RC Leiden, Netherlands Slotervaart Hosp, Fac Pharm & Pharmacol, NL-1066 EC Amsterdam, NetherlandsSlotervaart Hosp Amsterdam Netherlands NL-1066 EC Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 730, anno: 1999,
pagine: 95 - 99
SICI:
1387-2273(19990625)730:1<95:SLCAOP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Keywords:
derivatization, LC; pamidronate; 1-naphthylisothiocyanate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: Sparidans, RW Univ Utrecht, Dept Pharm, Sorbonnelaan 16, NL-3584 CA Utrecht, Netherlands Univ Utrecht Sorbonnelaan 16 Utrecht Netherlands NL-3584 CA
Citazione:
R.W. Sparidans et al., "Semi-automatic liquid chromatographic analysis of pamidronate in urine after derivatization with 1-naphthylisothiocyanate", J CHROMAT B, 730(1), 1999, pp. 95-99

Abstract

An existing sensitive chromatographic assay for pamidronate in urine has considerably been automated. Using the same sample processor, the solid-phase extraction (SPE) was automated separately from the derivatization with 1-naphthylisothiocyanate, the two-fold ion-pair Liquid-Liquid-extraction and the treatment with hydrogen peroxide for the 2-20 ng/ml concentration range. The automatic procedure was preceded by a triple calcium precipitation and interrupted by evaporation of the SPE eluate under nitrogen. For the 0.5-5 mu g/ml concentration range one automatic sequence was used by avoiding evaporation during the sample treatment. In addition to the labour-saving ofthe semi-automatic procedure, the daily sample-throughput was improved compared to the existing manual assay. Further, the validation showed marginalimprovements in the precision, accuracy and lower Limit of quantification. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:06:04