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Titolo:
High-performance liquid chromatographic analysis of physiological amino acids in human brain tumors by pre-column derivatization with phenylisothiocyanate
Autore:
Battaglia, A; Bertoluzza, A; Calbucci, F; Eusebi, V; Giorgianni, P; Ricci, R; Tosi, R; Tugnoli, V;
Indirizzi:
CNR, Ist Composti Carbonio Contenenti Eteroatom, I-40129 Bologna, Italy CNR Bologna Italy I-40129 o Contenenti Eteroatom, I-40129 Bologna, Italy Univ Bologna, Dip Biochim G Moruzzi, Sez Chim & Propedeut Biochim, I-40126Bologna, Italy Univ Bologna Bologna Italy I-40126 pedeut Biochim, I-40126Bologna, Italy Univ Bologna, Osped Bellaria, Div Neurochirurg, I-40139 Bologna, Italy Univ Bologna Bologna Italy I-40139 Neurochirurg, I-40139 Bologna, Italy Univ Bologna, Sez Anat Istol & Citol Patol M Malpighi, I-40139 Bologna, Italy Univ Bologna Bologna Italy I-40139 ol M Malpighi, I-40139 Bologna, Italy Osped Bellaria, Serv Neuroradiol, I-40139 Bologna, Italy Osped Bellaria Bologna Italy I-40139 Neuroradiol, I-40139 Bologna, Italy CNR, Inst Citomorfol Normale & Patol, I-40136 Bologna, Italy CNR BolognaItaly I-40136 morfol Normale & Patol, I-40136 Bologna, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 730, anno: 1999,
pagine: 81 - 93
SICI:
1387-2273(19990625)730:1<81:HLCAOP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECOLUMN DERIVATIZATION; RAT-BRAIN; SPECTROSCOPY; INVIVO; ELECTROPHORESIS; FLUORESCENCE; EXTRACTS; H-1-NMR; INVITRO; MRS;
Keywords:
derivatization, LC; amino acids; phenylisothiocyanate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Battaglia, A CNR, Ist Composti Carbonio Contenenti Eteroatom, Via Gobetti 101, I-40129 Bologna, Italy CNR Via Gobetti 101 Bologna Italy I-40129 129 Bologna, Italy
Citazione:
A. Battaglia et al., "High-performance liquid chromatographic analysis of physiological amino acids in human brain tumors by pre-column derivatization with phenylisothiocyanate", J CHROMAT B, 730(1), 1999, pp. 81-93

Abstract

A reversed-phase high-performance liquid chromatographic technique for thedetermination of free amino acids in five biopsies of human brain tumors (two meningiomas, one glioblastoma and two oligodendrogliomas) is described. The frozen tissues were homogenized, deproteinized with perchloric acid and neutralized with potassium hydroxide. Aliquots of the supernatant containing the physiological amino acids are used for pre-column derivatization with phenylisothiocyanate. The derivatized PTC-amino acids (phenylthiocarbamyl derivatives) are stable for a five day period if stored as a powder at -20 degrees C in an inert atmosphere and they can be analyzed on a reversed-phase column (PicoTag) using a gradient of two eluents with absorption detection at a wavelength of 254 nm. Good resolution of several amino acids (>30) is achieved within ca. 60 min. For most amino acids this method is suitable for an accurate measurement over a wide range of physiological concentrations (50-400 pmol) starting from a very small amount of sample. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 04/04/20 alle ore 02:29:30