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Titolo:
Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids
Autore:
Pineda, M; Fernandez, E; Torrents, D; Estevez, R; Lopez, C; Camps, M; Lloberas, J; Zorzano, A; Palacin, M;
Indirizzi:
Univ Barcelona, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 iol Mol, E-08028 Barcelona, Spain Univ Barcelona, Fac Biol, Dept Fisiol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 Fisiol, E-08028 Barcelona, Spain Univ Barcelona, Serv Cient Tecn, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 nt Tecn, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 28, volume: 274, anno: 1999,
pagine: 19738 - 19744
SICI:
0021-9258(19990709)274:28<19738:IOAMPL>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS-LAEVIS OOCYTES; ALANINE TRANSPORT; SURFACE-ANTIGEN; PLASMA-MEMBRANE; SYSTEM L; METABOLISM; CELLS; CLONING; CYSTINE; VESICLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Palacin, M Univ Barcelona, Dept Bioquim & Biol Mol, Avda Diagonal 645, E-08028 Barcelona, Spain Univ Barcelona Avda Diagonal 645 Barcelona Spain E-08028 Spain
Citazione:
M. Pineda et al., "Identification of a membrane protein, LAT-2, that co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids", J BIOL CHEM, 274(28), 1999, pp. 19738-19744

Abstract

We have identified a new human cDNA, L-amino acid transporter-2 (LAT-2), that induces a system L transport activity with 4F2hc (the heavy chain of the surface antigen 4F2, also named CD98) in oocytes, Human LAT-2 is the fourth member of the family of amino acid transporters that are subunits of 4F2hc, The amino acid transport activity induced by the co-expression of 4F2hcand LAT-2 was sodium-independent and showed broad specificity for small and large zwitterionic amino acids, as well as bulky analogs (e.g. BCH (2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid)). This transport activity was highly trans-stimulated, suggesting an exchanger mechanism of transport. Expression of tagged N-myc-LAT-2 alone in oocytes did not induce amino acid transport, and the protein had an intracellular location. Co-expression of N-myc-LAT-2 and 4F2hc gave amino acid transport induction and expression of N-myc-LAT-2 at the plasma membrane of the oocytes, These data suggest that LAT-S is an additional member of the family of 4F2 light chain subunits, which associates with 4F2hc to express a system L transport activity with broad specificity for zwitterionic amino acids, Human LAT-S mRNA is expressed in kidney >>> placenta >> brain, liver > spleen, skeletal muscle, heart, small intestine, and lung. Human LAT-2 gene localizes at chromosome 14q11.2-13 (13 cR or similar to 286 kb from marker D14S1349), The high expression ofLAT-S mRNA in epithelial cells of proximal tubules, the basolateral location of 4F2hc in these cells, and the amino acid transport activity of LAT-2 suggest that this transporter contributes to the renal reabsorption of neutral amino acids in the basolateral domain of epithelial proximal tubule cells.

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Documento generato il 28/01/21 alle ore 06:33:54