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Titolo:
Mycobacterium tuberculosis subsp caprae subsp nov.: a taxonomic study of anew member of the Mycobacterium tuberculosis complex isolated from goats in Spain
Autore:
Aranaz, A; Liebana, E; Gomez-Mampaso, E; Galan, JC; Cousins, D; Ortega, A; Blazquez, J; Baquero, F; Mateos, A; Suarez, G; Dominguez, L;
Indirizzi:
Univ Complutense Madrid, Fac Vet, Dept Patol Anim Sanida Anim 1, Madrid 28040, Spain Univ Complutense Madrid Madrid Spain 28040 a Anim 1, Madrid 28040, Spain Hosp Ramon y Cajal, Microbiol Serv, Madrid 28034, Spain Hosp Ramon y Cajal Madrid Spain 28034 icrobiol Serv, Madrid 28034, Spain Western Australia Dept Agr, Australian Reference Lab Bovine TB, S Perth, WA 6151, Australia Western Australia Dept Agr S Perth WA Australia 6151 , WA 6151, Australia Hosp Carlos 3, MP, Madrid 28029, Spain Hosp Carlos 3 Madrid Spain 28029Hosp Carlos 3, MP, Madrid 28029, Spain Ctr Nacl Invest Clin, Madrid 28029, Spain Ctr Nacl Invest Clin Madrid Spain 28029 Invest Clin, Madrid 28029, Spain
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
, volume: 49, anno: 1999,
parte:, 3
pagine: 1263 - 1273
SICI:
0020-7713(199907)49:<1263:MTSCSN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; DEOXYRIBONUCLEIC-ACID RELATEDNESS; FRAGMENT-LENGTH-POLYMORPHISM; SLOWLY-GROWING MYCOBACTERIA; CATALASE-PEROXIDASE GENE; BOVIS STRAINS; DNA AMPLIFICATION; SPECIES LEVEL; USEFUL TOOL; IDENTIFICATION;
Keywords:
Mycobacterium tuberculosis subsp caprae subsp nov.; Mycobacterium tuberculosis complex; tuberculosis; goats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Dominguez, L Univ Complutense Madrid, Fac Vet, Dept Patol Anim Sanida Anim1, Madrid 28040, Spain Univ Complutense Madrid Madrid Spain 28040 rid 28040, Spain
Citazione:
A. Aranaz et al., "Mycobacterium tuberculosis subsp caprae subsp nov.: a taxonomic study of anew member of the Mycobacterium tuberculosis complex isolated from goats in Spain", INT J SY B, 49, 1999, pp. 1263-1273

Abstract

Isolates from the Mycobacterium tuberculosis complex cultured from caprinepathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrate reduction and niacin accumulation, they weakly hydrolysed Tween 80, were sensitive to pyrazinamide (50 mu gml(-1)) and were resistant to 1 and 2 mu g tiophene-2-carboxylic acid hydrazide ml(-1) but not to 5 or 10 pg tiophene-2-carboxylic acid hydrazide ml(-1). Sequencing of the pncA gene revealed a polymorphism characteristic of M. tuberculosis, whereas oxyR, katG and gyrA sequences were characteristic of Mycobacterium bovis. The fingerprinting patterns obtained with IS6110 direct repeats and polymorphic G + C-rich sequence-associated RFLP and directvariable repeat-spacer oligonucelotide typing (spoligotyping) segregated these isolates from the other members of the complex. The results of this testing, together with the repeated association of this micro-organism with goats, suggest that st new member of this taxonomic complex not matching anyof the classical species had been identified. This unusual mycobacterium may play a role in the epidemiology of animal and human tuberculosis in Spain. The name Mycobacterium tuberculosis subsp. caprae subsp. nov. is proposed for these isolates. The type strain of Mycobacterium tuberculosis subsp. caprae subsp. nov. is gM-1(T)(= CIP 105776(T)).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:06:19