Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cryptobacterium curtum gen. nov., sp nov., a new genus of Gram-positive anaerobic rod isolated from human oral cavities
Autore:
Nakazawa, F; Poco, SE; Ikeda, T; Sato, M; Kalfas, S; Sundqvist, G; Hoshino, E;
Indirizzi:
Niigata Univ, Sch Dent, Dept Oral Microbiol, Niigata 9518514, Japan Niigata Univ Niigata Japan 9518514 ral Microbiol, Niigata 9518514, Japan Umea Univ, Sch Dent, Dept Oral Biol, Umea, Sweden Umea Univ Umea SwedenUmea Univ, Sch Dent, Dept Oral Biol, Umea, Sweden Umea Univ, Sch Dent, Dept Endodont, Umea, Sweden Umea Univ Umea SwedenUmea Univ, Sch Dent, Dept Endodont, Umea, Sweden
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
, volume: 49, anno: 1999,
parte:, 3
pagine: 1193 - 1200
SICI:
0020-7713(199907)49:<1193:CCGNSN>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDOMINANT OBLIGATE ANAEROBES; HUMAN PERIODONTAL POCKETS; EUBACTERIUM-NODATUM; SERUM ANTIBODIES; ADULT HUMANS; BACTERIOLOGY; PROTEINS; TIMIDUM; DENTIN; BRACHY;
Keywords:
Cryptobacterium curtum gen. nov.; sp nov.; DNA-DNA relatedness; 16S rDNA sequence; phylogenetic analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hoshino, E Niigata Univ, Sch Dent, Dept Oral Microbiol, Niigata 9518514, Japan Niigata Univ Niigata Japan 9518514 ol, Niigata 9518514, Japan
Citazione:
F. Nakazawa et al., "Cryptobacterium curtum gen. nov., sp nov., a new genus of Gram-positive anaerobic rod isolated from human oral cavities", INT J SY B, 49, 1999, pp. 1193-1200

Abstract

Novel Eubacterium-like isolates, strains 12-3(T) and KV43-B, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically andphylogenetically. The morphological and differential biochemical characteristics of these organisms are also described in this paper. These organismswere Cram-positive, anaerobic, non-spore-forming, rod-shaped bacteria thatwere inert in most of the conventional biochemical tests and closely resembled members of asaccharolytic oral Eubacterium species. On the other hand,protein profiles of whole cells in SOS-PAGE and Western immunoblotting reaction analysis distinguished these isolates from strains of the previously described genus Eubacterium. The G + C content of the DNAs from the novel isolates was 50 and 51 mol%, respectively. The levels of DNA-DNA relatednessto other asaccharolytic oral Eubacterium species, including Eubacterium brachy, Eubacterium lentum, Eubacterium nodatum, Eubacterium timidum, Eubacterium saphenum, Eubacterium minutum and Eubacterium exiguum, was less than 11 %. These organisms also exhibited a very low level of reassociation with the DNA of Eubacterium limosum, the type species of the genus Eubacterium. The results of 16S rDNA sequence comparisons revealed that these organisms represent a novel lineage distinct from all previously described genera of Gram-positive, rod-shaped bacteria. On the basis of our results, it is suggested that strains 12-3(T) and KV43-B should be classified in a new genus and species, for which the name Cryptobacterium curtum gen. nov., sp. nov. is proposed. The type strain of Cryptobacterium curtum is 12-3(T)(= ATCC 700683(T)).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:01:59