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Titolo:
An assay for the detection of xenoestrogens based on a promoter containingoverlapping EREs
Autore:
Massaad, C; Barouki, R;
Indirizzi:
Univ Paris 05, INSERM, Paris, France Univ Paris 05 Paris FranceUniv Paris 05, INSERM, Paris, France
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 7, volume: 107, anno: 1999,
pagine: 563 - 566
SICI:
0091-6765(199907)107:7<563:AAFTDO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPONSIVE ELEMENT; ESTROGEN-RECEPTOR; GENE-EXPRESSION; RETE TESTIS; SEMEN; CELLS; DIETHYLSTILBESTROL; SUPERFAMILY; QUALITY; BINDING;
Keywords:
detection assay; ERE; estrogen receptor; overlapping ERE; transcription; xenoestrogens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Barouki, R Ctr Univ St Peres, U490, INSERM, 45 Rue St Peres, F-75270 Paris06, France Ctr Univ St Peres 45 Rue St Peres Paris France 06 s 06, France
Citazione:
C. Massaad e R. Barouki, "An assay for the detection of xenoestrogens based on a promoter containingoverlapping EREs", ENVIR H PER, 107(7), 1999, pp. 563-566

Abstract

Xenoestrogens could be implicated in the decrease of male fertility and inthe increased incidence of testicular and breast cancers in humans. To predict their deleterious effects, various in vivo or in vitro tests have beenproposed to assay the xenoestrogenic activity. We have designed an assay for the detection of xenoestrogens based on a novel estrogen responsive unitformed by two overlapping estrogen response elements (overEREs). This construct is able to mediate a synergistic activation of transcription by 17 beta-estradiol. We have used the overERE unit to assay the estrogenic activity of synthetic compounds, mostly organochlorine compounds. By using the overERE construct, we were able to detect the estrogenic activity of compoundsat concentrations 10- to 100-fold lower than a single ERE (i.e., we detected the estrogenic effect of endosulfan at a concentration of 10(-5) M with ERE, whereas the overERE unit allowed us to detect a significant estrogenicactivity of endosulfan at a lower concentration (10(-6) M). Some compoundsdid not exhibit any estrogenic activity when tested with a classical ERE, whereas they were potent xenoestrogens when the overERE was used (i.e., Betanal). The assays we have developed are very sensitive and can be performedquickly. Moreover, because the promoter that we used contains only an overlapping ERE as a regulatory unit, the interference of the tested molecules with other regulatory pathways can be avoided.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:32:04