Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of Bartonella-specific immunodominant antigens recognized by the feline humoral immune system
Autore:
Freeland, RL; Scholl, DT; Rohde, KR; Shelton, LJ; OReilly, KL;
Indirizzi:
Louisiana State Univ, Sch Vet Med, Dept Vet Microbiol & Parasitol, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA Louisiana State Univ, Sch Vet Med, Dept Epidemiol & Community Hlth, Baton Rouge, LA 70803 USA Louisiana State Univ Baton Rouge LA USA 70803 , Baton Rouge, LA 70803 USA
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 4, volume: 6, anno: 1999,
pagine: 558 - 566
SICI:
1071-412X(199907)6:4<558:IOBIAR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAT-SCRATCH DISEASE; ROCHALIMAEA-HENSELAE; BACILLARY ANGIOMATOSIS; DOMESTIC CATS; EXPERIMENTAL-INFECTION; SEROLOGICAL RESPONSE; AFIPIA-FELIS; WESTERN-BLOT; RISK-FACTORS; QUINTANA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: O'Reilly, KL Louisiana State Univ, Sch Vet Med, Dept Vet Microbiol & Parasitol, S Stadium Dr, Baton Rouge, LA 70803 USA Louisiana State Univ S Stadium Dr Baton Rouge LA USA 70803 SA
Citazione:
R.L. Freeland et al., "Identification of Bartonella-specific immunodominant antigens recognized by the feline humoral immune system", CL DIAG LAB, 6(4), 1999, pp. 558-566

Abstract

The seroreactivities of both naturally and experimentally infected cats toBartonella henselae was examined. Serum samples collected weekly from ninecats experimentally infected with B. henselae LSU16 were tested by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. The magnitudeand isotype of the antibody response were investigated by ELISA, Western blot analysis allowed the identification of at least 24 Bartonella-specific antigens recognized by the cats during infection. Antibody titers to specific antigens, as determined by Western blot analysis, ranged from 10 to 640 and varied among the different antibody-antigen interactions. Absorption ofsera from an experimentally infected cat, using whole cells and cell lysates of various Bartonella species and other bacteria that commonly colonize cats, supported the identification of those Bartonella-specific antigens recognized by the experimentally infected cats. Furthermore, a number of possible species- and type-specific antigens were identified. Finally, sera obtained from cats at local animal shelters were screened for the presence of antibodies directed against the Bartonella-specific bands identified in theexperimentally infected cats. A number of Bartonella-specific antigens have been identified to which strong antibody responses are generated in both experimentally and naturally infected cats, some of which may be useful in diagnosing species- and/or type-specific infections. In addition, the results from these experiments will lead to the development of monoclonal antibodies targeted against those genus-, species-, and type-specific antigens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:19:57