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Titolo:
Determination of alkali and alkaline earth metal ions in blood serum by capillary electrophoresis with indirect UV detection
Autore:
Maruszak, W; Trojanowicz, M;
Indirizzi:
Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland Univ Warsaw Warsaw Poland PL-02093 w, Dept Chem, PL-02093 Warsaw, Poland
Titolo Testata:
CHEMIA ANALITYCZNA
fascicolo: 3, volume: 44, anno: 1999,
pagine: 437 - 453
SICI:
0009-2223(1999)44:3<437:DOAAAE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIRECT ULTRAVIOLET DETECTION; ZONE ELECTROPHORESIS; INORGANIC CATIONS; MIGRATION BEHAVIOR; REAL SAMPLES; SEPARATION; ELECTROLYTE; CHROMATOGRAPHY; MAGNESIUM; AMMONIUM;
Keywords:
capillary zone electrophoresis; alkali and alkaline earth metal ions; blood serum samples; supramolecular compounds;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Trojanowicz, M Univ Warsaw, Dept Chem, Pasteura 1, PL-02093 Warsaw, PolandUniv Warsaw Pasteura 1 Warsaw Poland PL-02093 rsaw, Poland
Citazione:
W. Maruszak e M. Trojanowicz, "Determination of alkali and alkaline earth metal ions in blood serum by capillary electrophoresis with indirect UV detection", CHEM ANAL, 44(3), 1999, pp. 437-453

Abstract

A capillary zone electrophoresis (CZE) method with indirect UV detection was developed for the fast and quantitative determination of alkali and alkaline earth metal ions in blood serum. Baseline separation of ammonium, potassium, sodium, calcium, magnesium and lithium cations was achieved within 7min. Carrier electrolyte consisted of 5 mmol l(-1) imidazole and 1 mmol l(-1) 18-crown-6 at pH 3.33. The effect of the pH and the concentration of electrolyte on the resolution and the efficiency of separation was investigated. Also, the effect of the addition of several supramolecular compounds such as cyclodextrins and crown ethers, to the electrolyte was studied. During the optimisation of the separation conditions two injection systems: hydrostatic and electrokinetic, were examined. The application of electrokinetic injection creates the described method fast and convenient, with good precision, for migration times from 0.4% to 0.7% and for signal of magnitude from 0.4% to 3.1%, high efficiency from 165000 to 5200000 theoretical platesand acceptable detection limits at submicromolar level. The described method was applied for the analysis of blood serum samples without the pretreatment procedure other than dilution. The quantitative results suggested thismethod to be useful for clinical applications.

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Documento generato il 18/01/20 alle ore 21:35:39