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Titolo:
Voltage-dependent K+ current in capillary endothelial cells isolated from guinea pig heart
Autore:
Dittrich, M; Daut, J;
Indirizzi:
Univ Marburg, Inst Normale & Pathol Physiol, D-35037 Marburg, Germany UnivMarburg Marburg Germany D-35037 l Physiol, D-35037 Marburg, Germany
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 1, volume: 46, anno: 1999,
pagine: H119 - H127
SICI:
0363-6135(199907)46:1<H119:VKCICE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; INTACT RAT AORTA; STIMULATED ENDOTHELIUM; ELECTRICAL-PROPERTIES; CALCIUM-CONCENTRATION; CYTOSOLIC CALCIUM; CHANNEL OPENERS; MEMBRANE; OSCILLATIONS; ACTIVATION;
Keywords:
coronary circulation; membrane potential oscillations; electrophysiology of endothelium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Dittrich, M Univ Marburg, Inst Normale & Pathol Physiol, Deutschhausstr 2,D-35037 Marburg, Germany Univ Marburg Deutschhausstr 2 Marburg Germany D-35037 Germany
Citazione:
M. Dittrich e J. Daut, "Voltage-dependent K+ current in capillary endothelial cells isolated from guinea pig heart", AM J P-HEAR, 46(1), 1999, pp. H119-H127

Abstract

Capillary fragments were isolated from guinea pig hearts, and their electrical properties were studied using the perforated-patch and cell-attached mode of the patch-clamp technique. A voltage-dependent K+ current was discovered that was activated at potentials positive to -20 mV and showed a sigmoid rising phase. For depolarizing voltage steps from -128 to +52 mV, the time to peak was 71 +/- 5 ms (mean +/- SE) and the amplitude of the current was 3.7 +/- 0.5 pA/pF in the presence of 5 mM external K+. The time course of inactivation was exponential with a time constant of 7.2 +/- 0.5 s at +52mV. The current was blocked by tetraethylammonium (inhibitory constant similar to 3 mM) but was not affected by charybdotoxin (1 mu M) or apamin (1 mu M) In the cell-attached mode, depolarization-activated single-channel currents were found that inactivated completely within 30 s; the single-channel conductance was 12.3 +/- 2.4 pS. The depolarization-activated K+ current described here may play a role in membrane potential oscillations of the endothelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 16:30:13