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Titolo:
Target selectivity of MAPK phosphorothioate antisense ODN on p42/p44, p38 MAPK, and JNK protein expression and its inhibitory effect on VSMC DNA synthesis
Autore:
Huang, SL; Ding, B; Li, J; Yi, FX; Liu, WL; Yu, QS; Guo, ZG;
Indirizzi:
Hu Nan Med Univ, Mol Pharmacol Lab, Changsha 410078, Peoples R China Hu Nan Med Univ Changsha Peoples R China 410078 410078, Peoples R China Guangzhou Med Coll, Guangzhou Res Inst Snake Venom, Guangzhou 510182, Peoples R China Guangzhou Med Coll Guangzhou Peoples R China 510182 182, Peoples R China
Titolo Testata:
ACTA PHARMACOLOGICA SINICA
fascicolo: 7, volume: 20, anno: 1999,
pagine: 613 - 617
SICI:
0253-9756(199907)20:7<613:TSOMPA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; KINASE; PREVENTION; RESTENOSIS; ACTIVATION;
Keywords:
protein kinases; antisense oligonucleotides; vascular smooth muscle; cultured cells; Western blotting; thymidine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Guo, ZG Hu Nan Med Univ, Mol Pharmacol Lab, Changsha 410078, Peoples R China Hu Nan Med Univ Changsha Peoples R China 410078 Peoples R China
Citazione:
S.L. Huang et al., "Target selectivity of MAPK phosphorothioate antisense ODN on p42/p44, p38 MAPK, and JNK protein expression and its inhibitory effect on VSMC DNA synthesis", ACT PHAR SI, 20(7), 1999, pp. 613-617

Abstract

AIM: To analyze the target selective and sequence specific inhibitory effect of mitogen-activated protein kinase (MAPK) phosphorothioate antisense oligodeoxynucleotides (ODN) on p42/p44, p38 MAPK, c-jun NH2-terminal protein kinases (JNK) protein expression, and DNA synthesis in vascular smooth muscle cell (VSMC). METHODS: Using a phosphorothioate-protected 17-mer antisense MAPK ODN directed against the initiation of translation sites of the p42/p44 MAPK isoforms by liposomal transfection to deplete cultured rat, rabbit, and fetal calf VSMC MAP kinases. The 17-mer sense and random sequence MAPK ODN were used as controls. After liposomal transfection, cells were exposed to 20% serum for 24 h, and then harvested in lysis buffer. P42/p44, p38 MAPK, and p46/p58 JNK protein expression were measured by Western blot. DNAsynthesis was measured by [H-3]thymidine incorporation. RESULTS: Treatmentwith MAPK antisense ODN (0.1-0.8 mu mol . L-1) for 48 h reduced phosphoredp42/p44 MAPK protein expression but without effect on p38 MAPK and JNK expression, and inhibited cultured rat, rabbit, and fetal calf VSMC [H-3]thymidine incorporation stimulated by 20% serum in a concentration-dependent manner. CONCLUSION: The MAPK antisense ODN target-selectively and sequence-specifically reduces the p42/p44 MAPK protein expression and concentration-dependently inhibits proliferation of rat, rabbit and fetal calf VSMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:24:59