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Titolo:
The human Prp8 protein is a component of both U2-and U12-dependent spliceosomes
Autore:
Luo, HBR; Moreau, GA; Levin, N; Moore, MJ;
Indirizzi:
Brandeis Univ, Dept Biochem, Howard Hughes Med Inst, Waltham, MA 02454 USABrandeis Univ Waltham MA USA 02454 Hughes Med Inst, Waltham, MA 02454 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 7, volume: 5, anno: 1999,
pagine: 893 - 908
SICI:
1355-8382(199907)5:7<893:THPPIA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN; CATALYTIC STEP-II; U5 SNRNP; SPLICING FACTOR; POLYMERASE-II; SACCHAROMYCES-CEREVISIAE; IN-VIVO; AT-AC; YEAST;
Keywords:
pre-mRNA splicing; Prp8p; spliceosome; U12-dependent spliceosome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Moore, MJ Brandeis Univ, Dept Biochem, Howard Hughes Med Inst, MS 009,415 South St, Waltham, MA 02454 USA Brandeis Univ MS 009,415 South St Waltham MA USA 02454 02454 USA
Citazione:
H.B.R. Luo et al., "The human Prp8 protein is a component of both U2-and U12-dependent spliceosomes", RNA, 5(7), 1999, pp. 893-908

Abstract

This study reports the cloning, sequencing, and development of antisera against the human U5 snRNP 220-kDa protein or hPrp8p. Prp8p is the most highly conserved large nuclear protein known to date, but it is not related to any other protein. Southern, Northern, and expressed sequence tag analyses indicate that hPrp8p is encoded by a single gene. Prp8p is a core component of U5 snRNP and the U4/U6.U5 tri-snRNP, and antibodies raised against it immunoprecipitate both the major, U2-dependent and minor, U12-dependent spliceosomes. These spliceosomes, which excise different classes of introns, contain distinct sets of snRNAs overlapping only with U5 snRNA. Other than thecore Sm proteins, hPrp8p is the first splicing factor shown to be common to both spliceosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:32:35