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Titolo:
Determination of optimal time for mating by artificial insemination with chilled semen using luteinizing hormone surge as an indicator in beagles
Autore:
Nishiyama, T; Kinugasa, T; Kimura, T; Watanabe, G; Taya, K; Tsumagari, S; Takeishi, M;
Indirizzi:
Nihon Univ, Coll Bioresource Sci, Lab Vet Obstet & Gynecol, Fujisawa, Kanagawa 2528510, Japan Nihon Univ Fujisawa Kanagawa Japan 2528510 isawa, Kanagawa 2528510, Japan Tokyo Univ Agr & Technol, Dept Vet Physiol, Fuchu, Tokyo 1830054, Japan Tokyo Univ Agr & Technol Fuchu Tokyo Japan 1830054 , Tokyo 1830054, Japan
Titolo Testata:
JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION
fascicolo: 4, volume: 35, anno: 1999,
pagine: 348 - 352
SICI:
0587-2871(199907/08)35:4<348:DOOTFM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROZEN; BITCH; OVULATION; PLASMA; FRESH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Nishiyama, T Nihon Univ, Coll Bioresource Sci, Lab Vet Obstet & Gynecol, 1866 Kameino, Fujisawa, Kanagawa 2528510, Japan Nihon Univ 1866 Kameino Fujisawa Kanagawa Japan 2528510 Japan
Citazione:
T. Nishiyama et al., "Determination of optimal time for mating by artificial insemination with chilled semen using luteinizing hormone surge as an indicator in beagles", J AM ANIM H, 35(4), 1999, pp. 348-352

Abstract

Artificial insemination (AI) was conducted using the second fraction of semen, which was collected from 15 male dogs, diluted to a total sperm count of 100 x 10(6) for each insemination with egg-yolk Tris (eyT) citrate acid buffer and incubated at 4 degrees C for 48 hours. Luteinizing hormone (LH) surge was detected to determine the optimal time for mating using canine LHassay kits. Artificial insemination using 100 x 10(6) sperm was performed on the fourth and sixth days or the fifth and seventh days after the LH surge. The conception rates were 33% (4/12) and 89% (8/9), respectively; the whelping rates also showed similar results. Serum LH and follicle stimulating hormone (FSH) concentrations were measured in nine dogs, and the mean LH concentration (+/-standard deviation) at LH surge was 15.77+/-7.66 ng/ml. The time of the LH surge detected by the canine LH assay kit was very similar to that: measured by radioimmunoassay (RIA).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:19:54